anti-Androgen Receptor antibody - ChIP-Grade

Key features and details

  • 产品描述: Goat Polyclonal antibody recognizes Androgen Receptor - ChIP-Grade
  • 反应物种: Hu
  • 预测物种: Ms, Rat, Cow, Dog, Pig
  • 应用: ChIP, ICC/IF, IHC-P, WB
  • 特异性: This antibody is expected to recognize isoform 1 (NP_000035.2) only.
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: Androgen Receptor
  • Brand:
CAT.NO. : ARG63608
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Product Details
概述
产品描述Goat Polyclonal antibody recognizes Androgen Receptor - ChIP-Grade
反应物种Hu
预测物种Ms, Rat, Cow, Dog, Pig
应用ChIP, ICC/IF, IHC-P, WB
特异性This antibody is expected to recognize isoform 1 (NP_000035.2) only.
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称Androgen Receptor
抗原物种Human
抗原EVQLGLGRVYPRPPSC
偶联标记Un-conjugated
別名TFM; Dihydrotestosterone receptor; Androgen receptor; KD; AR8; HUMARA; NR3C4; AIS; SBMA; HYSP1; SMAX1; Nuclear receptor subfamily 3 group C member 4; DHTR
应用说明
应用建议
应用推荐稀释比
ChIPAssay - dependent
ICC/IF10 µg/ml
IHC-P2 - 3 µg/ml
WB0.1 - 0.5 µg/ml
应用说明IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量~ 95 kDa
属性
形式Liquid
纯化Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
抗菌剂0.02% Sodium azide
稳定剂0.5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 367 Human AR

Swiss-port # P10275 Human Androgen receptor

基因名称AR
全名androgen receptor
背景介绍Androgen Receptor is a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (SBMA, also known as Kennedy's disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2017]
生物功能Androgen Receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen response elements/ARE on target genes, negatively regulating androgen receptor signaling and androgen-induced cell proliferation (PubMed:20812024). Transcription activation is also down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.

Isoform 3 and isoform 4 lack the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones. [UniProt]
研究领域Cancer antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody
预测分子量99 kDa
翻译后修饰Sumoylated on Lys-388 (major) and Lys-521. Ubiquitinated. Deubiquitinated by USP26. 'Lys-6' and 'Lys-27'-linked polyubiquitination by RNF6 modulates AR transcriptional activity and specificity.
Phosphorylated in prostate cancer cells in response to several growth factors including EGF. Phosphorylation is induced by c-Src kinase (CSK). Tyr-535 is one of the major phosphorylation sites and an increase in phosphorylation and Src kinase activity is associated with prostate cancer progression. Phosphorylation by TNK2 enhances the DNA-binding and transcriptional activity and may be responsible for androgen-independent progression of prostate cancer. Phosphorylation at Ser-83 by CDK9 regulates AR promoter selectivity and cell growth. Phosphorylation by PAK6 leads to AR-mediated transcription inhibition.
Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
检测图片 (5)
  • ARG63608 anti-Androgen Receptor antibody - ChIP-Grade ICC/IF image

    Immunofluorescence: Paraformaldehyde fixed MCF7 cells permeabilized with 0.15% Triton. Cells were stained with ARG63608 anti-Androgen Receptor antibody - ChIP-Grade (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.

  • ARG63608 anti-Androgen Receptor antibody - ChIP-Grade IHC-P image

    Immunohistochemistry: Paraffin-embedded Human prostate tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63608 anti-Androgen Receptor antibody (ChIP-Grade) at 2 µg/ml dilution followed by AP-staining.

    This picture shows nuclear staining in the secretory cells of the gland.

  • ARG63608 anti-Androgen Receptor antibody - ChIP-Grade WB image

    Western blot: 35 µg of Human cerebellum (A) and Human pancreas (B, negative control) lysates (in RIPA buffer) stained with ARG63608 anti-Androgen Receptor antibody - ChIP-Grade at 0.5 µg/ml (A) and 0.1 µg/ml (B) dilutions and incubated at RT for 1 hour.

  • ARG63608 anti-Androgen Receptor antibody - ChIP-Grade ChIP image

    ChIP: 2 µg ARG63608 anti-Androgen Receptor antibody (ChIP-Grade) with 1 µg DHT-treated HEC50 chromatin using the using the Chromatrap® spin column sonication kit (Protein G) measuring FKBP5 enrichment.

  • ARG63608 anti-Androgen Receptor antibody - ChIP-Grade ICC/IF image

    Immunofluorescence: Paraformaldehyde fixed U2OS cells permeabilized with 0.15% Triton. Cells were stained with ARG63608 anti-Androgen Receptor antibody - ChIP-Grade (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.

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