anti-ARC / NOL3 antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes ARC / NOL3
  • 反应物种: Hu, Ms
  • 应用: IP, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: ARC / NOL3
  • 抗原物种: Human
  • 抗原: Synthetic peptide around aa. 191-208 of Human ARC / NOL3. (PDPEPEPDFEERDESEDS)
  • Brand:
CAT.NO. : ARG56507
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes ARC / NOL3
反应物种Hu, Ms
应用IP, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称ARC / NOL3
抗原物种Human
抗原Synthetic peptide around aa. 191-208 of Human ARC / NOL3. (PDPEPEPDFEERDESEDS)
偶联标记Un-conjugated
別名Apoptosis repressor with CARD; MYP; Nucleolar protein of 30 kDa; ARC; Myp; Muscle-enriched cytoplasmic protein; Nop30; NOP; FCM; Nucleolar protein 3; NOP30
应用说明
应用建议
应用推荐稀释比
IPAssay-dependent
WB1:200
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Affinity purification with immunogen.
缓冲液PBS (pH 7.2) and 0.02% Sodium azide.
抗菌剂0.02% Sodium azide.
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 78688 Mouse NOL3

GeneID: 8996 Human NOL3

Swiss-port # O60936 Human Nucleolar protein 3

Swiss-port # Q9D1X0 Mouse Nucleolar protein 3

基因名称NOL3
全名nucleolar protein 3 (apoptosis repressor with CARD domain)
背景介绍This gene encodes an anti-apoptotic protein that has been shown to down-regulate the enzyme activities of caspase 2, caspase 8 and tumor protein p53. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
生物功能Isoform 1: May be involved in RNA splicing.
Isoform 2: Functions as an apoptosis repressor that blocks multiple modes of cell death. Inhibits extrinsic apoptotic pathways through two different ways. Firstly by interacting with FAS and FADD upon FAS activation blocking death-inducing signaling complex (DISC) assembly (By similarity). Secondly by interacting with CASP8 in a mitochondria localization- and phosphorylation-dependent manner, limiting the amount of soluble CASP8 available for DISC-mediated activation (By similarity). Inhibits intrinsic apoptotic pathway in response to a wide range of stresses, through its interaction with BAX resulting in BAX inactivation, preventing mitochondrial dysfunction and release of pro-apoptotic factors (PubMed:15004034). Inhibits calcium-mediated cell death by functioning as a cytosolic calcium buffer, dissociating its interaction with CASP8 and maintaining calcium homeostasis (PubMed:15509781). Negatively regulates oxidative stress-induced apoptosis by phosphorylation-dependent suppression of the mitochondria-mediated intrinsic pathway, by blocking CASP2 activation and BAX translocation (By similarity). Negatively regulates hypoxia-induced apoptosis in part by inhibiting the release of cytochrome c from mitochondria in a caspase-independent manner (By similarity). Also inhibits TNF-induced necrosis by preventing TNF-signaling pathway through TNFRSF1A interaction abrogating the recruitment of RIPK1 to complex I (By similarity). Finally through its role as apoptosis repressor, promotes vascular remodeling through inhibition of apoptosis and stimulation of proliferation, in response to hypoxia (By similarity). Inhibits too myoblast differentiation through caspase inhibition (By similarity). [UniProt]
细胞定位Isoform 1: Nucleolus.
Note: The SR-rich C-terminus mediates nuclear localization.

Isoform 2: Mitochondrion, Cytoplasm, Sarcoplasmic reticulum and Membrane.
Note: Phosphorylation at Thr-149 results in translocation to mitochondria. Colocalized with mitochondria in response to oxidative stress. [UniProt]
产品亮点Related products:
ARC antibodies; ARC ELISA Kits; Anti-Rabbit IgG secondary antibodies;
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预测分子量23 kDa
翻译后修饰Phosphorylation at Thr-149 is required for its antiapoptotic effect by blocking death-inducing signaling complex death-inducing signaling complex (DISC) activity through the control of interaction with CASP8. Phosphorylation at Thr-149 results in translocation to mitochondria and this translocation enables the binding to CASP8. Dephosphorylated at Thr-149 by calcineurin; doesn't inhibit the association between FADD and CASP8 and the consequent apoptosis.

Polyubiquitinated by MDM2; promoting proteasomal-dependent degradation in response to apoptotic stimuli. [UniProt]
检测图片 (1)
  • ARG56507 anti-ARC / NOL3 antibody WB image

    Western blot: 1) Mouse cerebrum, 2) Mouse liver, 3) Mouse heart, 4) Mouse smooth muscle, and 5) Mouse lung stained with ARG56507 anti-ARC / NOL3 antibody.

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