anti-ARPC2 antibody
Key features and details
- 产品描述:
- 反应物种:
- 预测物种:
- 应用:
- 特异性:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
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Brand:
Product Details
| 产品描述 | Goat Polyclonal antibody recognizes ARPC2 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Cow, Dog |
| 应用 | ICC/IF, IHC-P, WB |
| 特异性 | The reported variants NP_005722.1 and NP_690601.1 represent identical protein. |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ARPC2 |
| 抗原物种 | Human |
| 抗原 | C-EMKTITGKTFSSR |
| 偶联标记 | Un-conjugated |
| 別名 | Arp2/3 complex 34 kDa subunit; PNAS-139; p34-ARC; Actin-related protein 2/3 complex subunit 2; ARC34; p34-Arc; PRO2446 |
| 应用建议 |
| ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | Swiss-port # O15144 Human Actin-related protein 2/3 complex subunit 2 |
|---|---|
| 背景介绍 | This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p34 subunit, has yet to be determined. Two alternatively spliced variants have been characterized to date. Additional alternatively spliced variants have been described but their full length nature has not been determined. [provided by RefSeq, Jul 2008] |
| 研究领域 | Signaling Transduction antibody |
| 预测分子量 | 34 kDa |
ARG63465 anti-ARPC2 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed A431 cells permeabilized with 0.15% Triton. Cells were stained with ARG63465 anti-ARPC2 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
ARG63465 anti-ARPC2 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Spleen. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63465 anti-ARPC2 antibody at 3.8 µg/ml dilution followed by AP-staining.
ARG63465 anti-ARPC2 antibody WB image
Western blot: 35 µg of Caco-2 (A), HeLa (B) and Jurkat (C) cell lysates (in RIPA buffer) stained with ARG63465 anti-ARPC2 antibody at 0.5 µg/ml (A) and 0.1 µg/ml (B, C) dilutions and incubated at RT for 1 hour.
ARG63465 anti-ARPC2 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG63465 anti-ARPC2 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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