anti-Aryl Hydrocarbon Receptor antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes Aryl Hydrocarbon Receptor
  • 反应物种: Hu, Ms, Rat
  • 应用: FACS, IHC-P
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: Aryl Hydrocarbon Receptor
  • 抗原物种: Human
  • 抗原: Synthetic peptide corresponding to a sequence of Human Aryl Hydrocarbon Receptor. (AFLNKFQNGVLNETYPAELNNINNTQTTTHLQPLHH)
  • Brand:
CAT.NO. : ARG41570
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes Aryl Hydrocarbon Receptor
反应物种Hu, Ms, Rat
应用FACS, IHC-P
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称Aryl Hydrocarbon Receptor
抗原物种Human
抗原Synthetic peptide corresponding to a sequence of Human Aryl Hydrocarbon Receptor. (AFLNKFQNGVLNETYPAELNNINNTQTTTHLQPLHH)
偶联标记Un-conjugated
別名Aryl hydrocarbon receptor; AhR; Ah receptor; bHLHe76; Class E basic helix-loop-helix protein 76
应用说明
应用建议
应用推荐稀释比
FACS1:150 - 1:500
IHC-P1:200 - 1:1000
应用说明IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Affinity purification with immunogen.
缓冲液0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose.
抗菌剂0.05% Sodium azide
稳定剂4% Trehalose
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 11622 Mouse AHR

GeneID: 196 Human AHR

GeneID: 25690 Rat AHR

基因名称AHR
全名aryl hydrocarbon receptor
背景介绍The protein encoded by this gene is a ligand-activated helix-loop-helix transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Before ligand binding, the encoded protein is sequestered in the cytoplasm; upon ligand binding, this protein moves to the nucleus and stimulates transcription of target genes. [provided by RefSeq, Sep 2015]
生物功能Ligand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates. Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Involved in cell-cycle regulation. Likely to play an important role in the development and maturation of many tissues. Regulates the circadian clock by inhibiting the basal and circadian expression of the core circadian component PER1. Inhibits PER1 by repressing the CLOCK-ARNTL/BMAL1 heterodimer mediated transcriptional activation of PER1. [UniProt]
细胞定位Cytoplasm. Nucleus. Note=Initially cytoplasmic; upon binding with ligand and interaction with a HSP90, it translocates to the nucleus. [UniProt]
预测分子量96 kDa
检测图片 (9)
  • ARG41570 anti-Aryl Hydrocarbon Receptor antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41570 anti-Aryl Hydrocarbon Receptor antibody at 2 µg/ml dilution, overnight at 4°C.

  • ARG41570 anti-Aryl Hydrocarbon Receptor antibody FACS image

    Flow Cytometry: U87 cells were blocked with 10% normal Goat serum and then stained with ARG41570 anti-Aryl Hydrocarbon Receptor antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.

  • ARG41570 anti-Aryl Hydrocarbon Receptor antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human cholangiocarcinoma tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41570 anti-Aryl Hydrocarbon Receptor antibody at 2 µg/ml dilution, overnight at 4°C.

  • ARG41570 anti-Aryl Hydrocarbon Receptor antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human oesophagus squama cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41570 anti-Aryl Hydrocarbon Receptor antibody at 2 µg/ml dilution, overnight at 4°C.

  • ARG41570 anti-Aryl Hydrocarbon Receptor antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human tonsil tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41570 anti-Aryl Hydrocarbon Receptor antibody at 2 µg/ml dilution, overnight at 4°C.

  • ARG41570 anti-Aryl Hydrocarbon Receptor antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse liver tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41570 anti-Aryl Hydrocarbon Receptor antibody at 2 µg/ml dilution, overnight at 4°C.

  • ARG41570 anti-Aryl Hydrocarbon Receptor antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat small intestine tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41570 anti-Aryl Hydrocarbon Receptor antibody at 2 µg/ml dilution, overnight at 4°C.

  • ARG41570 anti-Aryl Hydrocarbon Receptor antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat spleen tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41570 anti-Aryl Hydrocarbon Receptor antibody at 2 µg/ml dilution, overnight at 4°C.

  • ARG41570 anti-Aryl Hydrocarbon Receptor antibody FACS image

    Flow Cytometry: U937 cells were blocked with 10% normal Goat serum and then stained with ARG41570 anti-Aryl Hydrocarbon Receptor antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.

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