anti-Aspartate Aminotransferase antibody [3G11]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [3G11] recognizes Aspartate Aminotransferase
  • 反应物种: Hu
  • 应用: FACS, WB
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: 3G11
  • 同位型: IgG2a, kappa
  • 靶点名称: Aspartate Aminotransferase
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG57174
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Product Details
概述
产品描述Mouse Monoclonal antibody [3G11] recognizes Aspartate Aminotransferase
反应物种Hu
应用FACS, WB
宿主Mouse
克隆Monoclonal
克隆号3G11
同位型IgG2a, kappa
靶点名称Aspartate Aminotransferase
抗原物种Human
抗原Recombinant fragment around aa. 1-413 of Human Aspartate Aminotransferase
偶联标记Un-conjugated
別名Cysteine transaminase, cytoplasmic; cAspAT; GIG18; Glutamate oxaloacetate transaminase 1; cCAT; EC 2.6.1.3; Cysteine aminotransferase, cytoplasmic; ASTQTL1; AST1; EC 2.6.1.1; Transaminase A; Aspartate aminotransferase, cytoplasmic
应用说明
应用建议
应用推荐稀释比
FACSAssay-dependent
WBAssay-dependent
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purification with Protein A.
缓冲液PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
抗菌剂0.02% Sodium azide
稳定剂10% Glycerol
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 2805 Human GOT1

Swiss-port # P17174 Human Aspartate aminotransferase, cytoplasmic

基因名称GOT1
全名glutamic-oxaloacetic transaminase 1, soluble
背景介绍Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008]
生物功能Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. [UniProt]
预测分子量46 kDa
检测图片 (5)
  • ARG57174 anti-Aspartate Aminotransferase antibody [3G11] FACS image

    Flow Cytometry: Hep3B cell line stained with ARG57174 anti-Aspartate Aminotransferase antibody [3G11] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody: Mouse IgG (black line).

  • ARG57174 anti-Aspartate Aminotransferase antibody [3G11] WB image

    Western blot: 40 µg of A427 cell lysate stained with ARG57174 anti-Aspartate Aminotransferase antibody [3G11] at 1:1000.

  • ARG57174 anti-Aspartate Aminotransferase antibody [3G11] FACS image

    Flow Cytometry: HeLa cell line stained with ARG57174 anti-Aspartate Aminotransferase antibody [3G11] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody: Mouse IgG (black line).

  • ARG57174 anti-Aspartate Aminotransferase antibody [3G11] WB image

    Western blot: 100 ng of 1) Recombinant Human GOT1 protein, and 2) Recombinant Human GOT2 protein stained with ARG57174 anti-Aspartate Aminotransferase antibody [3G11] at 1:1000.

  • ARG57174 anti-Aspartate Aminotransferase antibody [3G11] WB image

    Western blot: 10 µg of 1) 293T, and 2) GOT1 Transfected 293T cell lysate stained with ARG57174 anti-Aspartate Aminotransferase antibody [3G11] at 1:1000.

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