anti-Aspartate Aminotransferase antibody [3G11]

CAT.NO. : ARG57174

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Size:

50 μl

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

概述

产品描述	Mouse Monoclonal antibody [3G11] recognizes Aspartate Aminotransferase
反应物种	Hu
应用	FACS, WB
宿主	Mouse
克隆	Monoclonal
克隆号	3G11
同位型	IgG2a, kappa
靶点名称	Aspartate Aminotransferase
抗原物种	Human
抗原	Recombinant fragment around aa. 1-413 of Human Aspartate Aminotransferase
偶联标记	Un-conjugated
別名	Cysteine transaminase, cytoplasmic; cAspAT; GIG18; Glutamate oxaloacetate transaminase 1; cCAT; EC 2.6.1.3; Cysteine aminotransferase, cytoplasmic; ASTQTL1; AST1; EC 2.6.1.1; Transaminase A; Aspartate aminotransferase, cytoplasmic

应用说明

应用建议

应用	推荐稀释比
FACS	Assay-dependent
WB	Assay-dependent

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein A.
缓冲液	PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	10% Glycerol
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 2805 Human GOT1 Swiss-port # P17174 Human Aspartate aminotransferase, cytoplasmic
基因名称	GOT1
全名	glutamic-oxaloacetic transaminase 1, soluble
背景介绍	Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008]
生物功能	Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. [UniProt]
预测分子量	46 kDa

检测图片 (5)

ARG57174 anti-Aspartate Aminotransferase antibody [3G11] FACS image
Flow Cytometry: Hep3B cell line stained with ARG57174 anti-Aspartate Aminotransferase antibody [3G11] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody: Mouse IgG (black line).
ARG57174 anti-Aspartate Aminotransferase antibody [3G11] WB image
Western blot: 40 µg of A427 cell lysate stained with ARG57174 anti-Aspartate Aminotransferase antibody [3G11] at 1:1000.
ARG57174 anti-Aspartate Aminotransferase antibody [3G11] FACS image
Flow Cytometry: HeLa cell line stained with ARG57174 anti-Aspartate Aminotransferase antibody [3G11] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody: Mouse IgG (black line).
ARG57174 anti-Aspartate Aminotransferase antibody [3G11] WB image
Western blot: 100 ng of 1) Recombinant Human GOT1 protein, and 2) Recombinant Human GOT2 protein stained with ARG57174 anti-Aspartate Aminotransferase antibody [3G11] at 1:1000.
ARG57174 anti-Aspartate Aminotransferase antibody [3G11] WB image
Western blot: 10 µg of 1) 293T, and 2) GOT1 Transfected 293T cell lysate stained with ARG57174 anti-Aspartate Aminotransferase antibody [3G11] at 1:1000.

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