anti-Aspartate Aminotransferase antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
CAT.NO. : ARG41581
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Aspartate Aminotransferase |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Aspartate Aminotransferase |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 244-413 of Human Aspartate Aminotransferase (NP_002070.1). |
| 偶联标记 | Un-conjugated |
| 別名 | Cysteine transaminase, cytoplasmic; cAspAT; GIG18; Glutamate oxaloacetate transaminase 1; cCAT; EC 2.6.1.3; Cysteine aminotransferase, cytoplasmic; ASTQTL1; AST1; EC 2.6.1.1; Transaminase A; Aspartate aminotransferase, cytoplasmic |
应用说明
| 应用建议 |
| ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | HepG2 | ||||||||
| 实际分子量 | ~ 43 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | GOT1 |
| 全名 | glutamic-oxaloacetic transaminase 1, soluble |
| 背景介绍 | Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008] |
| 生物功能 | Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 预测分子量 | 46 kDa |
检测图片 (3)
ARG41581 anti-Aspartate Aminotransferase antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG41581 anti-Aspartate Aminotransferase antibody.
ARG41581 anti-Aspartate Aminotransferase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue stained with ARG41581 anti-Aspartate Aminotransferase antibody at 1:100 dilution.
ARG41581 anti-Aspartate Aminotransferase antibody WB image
Western blot: 25 µg of HepG2 cell lysate stained with ARG41581 anti-Aspartate Aminotransferase antibody at 1:1000 dilution.
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