anti-ATF4 antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes ATF4
  • 反应物种: Hu, Ms, Rat
  • 应用: ELISA, FACS, ICC/IF, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: ATF4
  • 抗原物种: Human
  • 抗原: Human ATF4 recombinant protein
  • Brand:
CAT.NO. : ARG43903
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes ATF4
反应物种Hu, Ms, Rat
应用ELISA, FACS, ICC/IF, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称ATF4
抗原物种Human
抗原Human ATF4 recombinant protein
表达系统E.coli
偶联标记Un-conjugated
Protein Full nameCyclic AMP-dependent transcription factor ATF-4
別名ATF4; Activating Transcription Factor 4; CREB-2; TAXREB67; TXREB; Tax-Responsive Enhancer Element-Binding Protein 67; Cyclic AMP-Dependent Transcription Factor ATF-4; Cyclic AMP-Responsive Element-Binding Protein 2; CAMP-Dependent Transcription Factor ATF-4; CAMP-Responsive Element-Binding Protein 2; Tax-Responsive Enhancer Element B67; CREB2; Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67); CAMP Response Element-Binding Protein 2; DNA-Binding Protein TAXREB67; TaxREB67
应用说明
应用建议
应用推荐稀释比
ELISA0.1-0.5 µg/ml
FACS1-3 µg/1x10^6 cells
ICC/IF5 µg/ml
WB0.25-0.5 µg/ml
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
缓冲液0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose.
稳定剂4% Trehalose
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
生物信息
数据库连接

GeneID: 11911 Mouse ATF4

GeneID: 468 Human ATF4

GeneID: 79255 Rat ATF4

基因名称ATF4
全名Activating Transcription Factor 4
背景介绍This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. [provided by RefSeq, Sep 2011]
生物功能Transcription factor that binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3') and displays two biological functions, as regulator of metabolic and redox processes under normal cellular conditions, and as master transcription factor during integrated stress response (ISR).
细胞定位Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Nucleus
预测分子量39 kDa
翻译后修饰Acetylation, Hydroxylation, Isopeptide bond, Phosphoprotein, Ubl conjugation
检测图片 (5)
  • ARG43903 anti-ATF4 antibody ICC/IF image

    Immunofluorescence: Hela cells stained with ARG43903 anti-ATF4 antibody antibody at 5 μg/ml dilution.

  • ARG43903 anti-ATF4 antibody WB image

    Western blot: HepG2 stained with ARG43903 anti-ATF4 antibody at 0.5 μg/mL dilution.

  • ARG43903 anti-ATF4 antibody FACS image

    Flow Cytometry: SiHa cells stained with ARG43903 anti-ATF4 antibody (blue) at 1 μg/1x10^6 cells dilution.

  • ARG43903 anti-ATF4 antibody WB image

    Western blot: Rat brain stained with ARG43903 anti-ATF4 antibody at 0.5 μg/mL dilution.

  • ARG43903 anti-ATF4 antibody WB image

    Western blot: Mouse brain stained with ARG43903 anti-ATF4 antibody at 0.5 μg/mL dilution.

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