anti-ATG7 antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
-
Brand:
CAT.NO. : ARG54916
US＄ Please choose
US＄ Please choose
Size:
Trail, Bulk size or Custom requests Please contact us
*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。
Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ATG7 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ELISA, ICC/IF, WB |
| 特异性 | At least three isoforms of APG7 are known to exist; this antibody will detect all three isoforms. APG7 antibody is predicted not to cross-react with other ATG family proteins. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ATG7 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide (17 aa) within aa. 590-640 of Human APG7. |
| 偶联标记 | Un-conjugated |
| 別名 | Ubiquitin-like modifier-activating enzyme ATG7; hAGP7; Autophagy-related protein 7; GSA7; Ubiquitin-activating enzyme E1-like protein; APG7-LIKE; APG7L; APG7-like; ATG12-activating enzyme E1 ATG7 |
应用说明
| 应用建议 |
| ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Caco-2 Cell Lysate |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # O95352 Human Ubiquitin-like modifier-activating enzyme ATG7 Swiss-port # Q9D906 Mouse Ubiquitin-like modifier-activating enzyme ATG7 |
|---|---|
| 基因名称 | ATG7 |
| 全名 | autophagy related 7 |
| 背景介绍 | APG7 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme; this function is conserved in the mammalian homolog. In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles. It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death. |
| 生物功能 | E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation. [UniProt] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 78 kDa |
| 翻译后修饰 | Acetylated by EP300. |
检测图片 (3)
ARG54916 anti-ATG7 antibody WB image
Western blot: Caco-2 cell lysate stained with ARG54916 anti-ATG7 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml dilution.
ARG54916 anti-ATG7 antibody ICC/IF image
Immunocytochemistry: MCF7 cells stained with ARG54916 anti-ATG7 antibody at 10 ug/ml dilution.
ARG54916 anti-ATG7 antibody ICC/IF image
Immunofluorescence: MCF7 cells stained with ARG54916 anti-ATG7 antibody at 20 ug/ml dilution.
New Products
