anti-ATP Citrate Lyase antibody [5I2]

Key features and details

产品描述: Mouse Monoclonal antibody [5I2] recognizes ATP Citrate Lyase
反应物种: Hu, Ms, Rat
应用: FACS, ICC/IF, IHC-P, WB
宿主: Mouse
克隆: Monoclonal
克隆号: 5I2
同位型: IgG2b
靶点名称: ATP Citrate Lyase
抗原物种: Human

Brand:

CAT.NO. : ARG42696

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Size:

50 μg

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*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

概述

产品描述	Mouse Monoclonal antibody [5I2] recognizes ATP Citrate Lyase
反应物种	Hu, Ms, Rat
应用	FACS, ICC/IF, IHC-P, WB
宿主	Mouse
克隆	Monoclonal
克隆号	5I2
同位型	IgG2b
靶点名称	ATP Citrate Lyase
抗原物种	Human
抗原	Recombinant protein corresponding to M1-I180 of Human ATP Citrate Lyase.
偶联标记	Un-conjugated
別名	ACL; ATP-citrate synthase; Citrate cleavage enzyme; CLATP; EC 2.3.3.8; pro-S-; ATP-citrate; ATPCL

应用说明

应用建议

应用	推荐稀释比
FACS	1:150 - 1:500
ICC/IF	1:200 - 1:1000
IHC-P	1:200 - 1:1000
WB	1:500 - 1:2000

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

实际分子量

~ 120 kDa

属性

形式	Liquid
纯化	Affinity purification with immunogen.
缓冲液	0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose.
抗菌剂	0.05% Sodium azide
稳定剂	4% Trehalose
浓度	0.5 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 104112 Mouse ACLY GeneID: 24159 Rat ACLY GeneID: 47 Human ACLY Swiss-port # P16638 Rat ATP-citrate synthase Swiss-port # P53396 Human ATP-citrate synthase Swiss-port # Q91V92 Mouse ATP-citrate synthase More
基因名称	ACLY
全名	ATP citrate lyase
背景介绍	ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014]
生物功能	Catalyzes the cleavage of citrate into oxaloacetate and acetyl-CoA, the latter serving as common substrate for de novo cholesterol and fatty acid synthesis. [UniProt]
细胞定位	Cytoplasm. [UniProt]
预测分子量	121 kDa
翻译后修饰	ISGylated. Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2. Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site (Probable). [UniProt]

检测图片 (8)

ARG42696 anti-ATP Citrate Lyase antibody [5I2] ICC/IF image
Immunofluorescence: MCF7 cells were blocked with 10% goat serum and then stained with ARG42696 anti-ATP Citrate Lyase antibody [5I2] (green) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
ARG42696 anti-ATP Citrate Lyase antibody [5I2] IHC-P image
Immunohistochemistry: Paraffin-embedded Human pancreatic cancer tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42696 anti-ATP Citrate Lyase antibody [5I2] at 1 µg/ml dilution, overnight at 4°C.
ARG42696 anti-ATP Citrate Lyase antibody [5I2] WB image
Western blot: 50 µg of samples under reducing condition. Human placenta, U-87MG, HEK293, Caco-2, HL-60, Raji, THP-1 and PANC-1 whole cell lysates stained with ARG42696 anti-ATP Citrate Lyase antibody [5I2] at 0.5 µg/ml dilution, overnight at 4°C.
ARG42696 anti-ATP Citrate Lyase antibody [5I2] FACS image
Flow Cytometry: A549 cells were blocked with 10% normal goat serum and then stained with ARG42696 anti-ATP Citrate Lyase antibody [5I2] (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was mouse IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
ARG42696 anti-ATP Citrate Lyase antibody [5I2] IHC-P image
Immunohistochemistry: Paraffin-embedded Human testis cancer tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42696 anti-ATP Citrate Lyase antibody [5I2] at 1 µg/ml dilution, overnight at 4°C.
ARG42696 anti-ATP Citrate Lyase antibody [5I2] IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse pancreas tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42696 anti-ATP Citrate Lyase antibody [5I2] at 1 µg/ml dilution, overnight at 4°C.
ARG42696 anti-ATP Citrate Lyase antibody [5I2] IHC-P image
Immunohistochemistry: Paraffin-embedded Rat pancreas tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42696 anti-ATP Citrate Lyase antibody [5I2] at 1 µg/ml dilution, overnight at 4°C.
ARG42696 anti-ATP Citrate Lyase antibody [5I2] WB image
Western blot: 50 µg of samples under reducing condition. Rat lung, Rat testis, Rat kidney, Rat brain, Mouse lung, Mouse testis, Mouse kidney and Mouse brain lysates stained with ARG42696 anti-ATP Citrate Lyase antibody [5I2] at 0.5 µg/ml dilution, overnight at 4°C.