anti-ATP1A / Na+ K+ ATPase alpha antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
Product Details
| 产品描述 | Rabbit Polyclonal antibody recognizes ATP1A / Na+ K+ ATPase alpha |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | IHC-P, IP, WB |
| 特异性 | This antibody is predicted to react with ATP1A1, ATP1A2, ATP1A3 and ATP1A4. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ATP1A / Na+ K+ ATPase alpha |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 551-850 of Human ATP1A1 (NP_000692.2). |
| 偶联标记 | Un-conjugated |
| 別名 | Sodium pump subunit alpha; Sodium/potassium-transporting ATPase subunit alpha |
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | HeLa | ||||||||
| 实际分子量 | ~ 113kDa |
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | Swiss-port # P05023 Human Sodium/potassium-transporting ATPase subunit alpha-1 Swiss-port # Q8VDN2 Mouse Sodium/potassium-transporting ATPase subunit alpha-1 |
|---|---|
| 基因名称 | ATP1A |
| 全名 | ATPase, Na+/K+ transporting, alpha |
| 背景介绍 | ATP1A protein belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009] |
| 生物功能 | ATP1A is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. [UniProt] |
| 细胞定位 | Cell membrane, Multi-pass membrane protein, Melanosome. [UniProt] |
| 预测分子量 | 113 kDa |
| 翻译后修饰 | Phosphorylation on Tyr-10 modulates pumping activity. Phosphorylation of Ser-943 by PKA modulates the response of ATP1A1 to PKC. Dephosphorylation by protein phosphatase 2A (PP2A) following increases in intracellular sodium, leading to increase catalytic activity (By similarity). [UniProt] |
ARG41952 anti-ATP1A / Na+ K+ ATPase alpha antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human prostate tissue stained with ARG41952 anti-ATP1A / Na+ K+ ATPase alpha antibody at 1:100 dilution.
ARG41952 anti-ATP1A / Na+ K+ ATPase alpha antibody WB image
Western blot: 25 µg of HeLa whole cell lysate stained with ARG41952 anti-ATP1A / Na+ K+ ATPase alpha antibody at 1:500 dilution.
ARG41952 anti-ATP1A / Na+ K+ ATPase alpha antibody IP image
Immunoprecipitation: 200 µg extracts of LO2 cells were immunoprecipitated and stained with ARG41952 anti-ATP1A / Na+ K+ ATPase alpha antibody at 1:1000 dilution.
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