anti-ATP5B antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes ATP5B
  • 反应物种: Hu, Ms, Rat
  • 应用: ICC/IF, IHC-P, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: ATP5B
  • 抗原物种: Human
  • 抗原: Recombinant fusion protein corresponding to aa. 230-529 of Human ATP5B (NP_001677.2).
  • Brand:
CAT.NO. : ARG40752
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes ATP5B
反应物种Hu, Ms, Rat
应用ICC/IF, IHC-P, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称ATP5B
抗原物种Human
抗原Recombinant fusion protein corresponding to aa. 230-529 of Human ATP5B (NP_001677.2).
偶联标记Un-conjugated
別名ATPMB; ATPSB; EC 3.6.3.14; HEL-S-271; ATP synthase subunit beta, mitochondrial
应用说明
应用建议
应用推荐稀释比
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照HepG2
实际分子量56 kDa
属性
形式Liquid
纯化Affinity purified.
缓冲液PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
抗菌剂0.02% Sodium azide
稳定剂50% Glycerol
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 11947 Mouse ATP5B

GeneID: 171374 Rat ATP5B

GeneID: 506 Human ATP5B

基因名称ATP5B
全名ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
背景介绍This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core. [provided by RefSeq, Jul 2008]
生物功能Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. [UniProt]
细胞定位Mitochondrion inner membrane; Peripheral membrane protein; Matrix side. [UniProt]
预测分子量57 kDa
检测图片 (3)
  • ARG40752 anti-ATP5B antibody ICC/IF image

    Immunofluorescence: NIH/3T3 cells stained with ARG40752 anti-ATP5B antibody at 1:100 dilution.

  • ARG40752 anti-ATP5B antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse heart stained with ARG40752 anti-ATP5B antibody at1:100 dilution.

  • ARG40752 anti-ATP5B antibody WB image

    Western blot: 25 µg of HepG2 cell lysate stained with ARG40752 anti-ATP5B antibody at 1:1000 dilution.

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