anti-ATP5B antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes ATP5B
  • 反应物种: Hu
  • 预测物种: Ms, Rat, Bov
  • 应用: FACS, ICC/IF, IHC-P, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: ATP5B
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG55558
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes ATP5B
反应物种Hu
预测物种Ms, Rat, Bov
应用FACS, ICC/IF, IHC-P, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称ATP5B
抗原物种Human
抗原KLH-conjugated synthetic peptide corresponding to aa. 135-163 (Center) of Human ATP5B.
偶联标记Un-conjugated
別名ATPMB; ATPSB; EC 3.6.3.14; HEL-S-271; ATP synthase subunit beta, mitochondrial
应用说明
应用建议
应用推荐稀释比
FACS1:10 - 1:50
ICC/IF1:25
IHC-P1:50 - 1:100
WB1:1000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照WiDr
属性
形式Liquid
纯化Purification with Protein A and immunogen peptide.
缓冲液PBS and 0.09% (W/V) Sodium azide
抗菌剂0.09% (W/V) Sodium azide
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 506 Human ATP5B

Swiss-port # P06576 Human ATP synthase subunit beta, mitochondrial

基因名称ATP5B
全名ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
背景介绍This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core. [provided by RefSeq, Jul 2008]
生物功能Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. [UniProt]
细胞定位Mitochondrion. Mitochondrion inner membrane. Note=Peripheral membrane protein
研究领域Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
预测分子量57 kDa
检测图片 (4)
  • ARG55558 anti-ATP5B antibody ICC/IF image

    Immunofluorescence: SK-BR-3 cells stained with ARG55558 anti-ATP5B antibody (green) at 1:25 dilution. DAPI (blue) for nuclear staining.

  • ARG55558 anti-ATP5B antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human liver section stained with ARG55558 anti-ATP5B antibody at 1:25 dilution.

  • ARG55558 anti-ATP5B antibody WB image

    Western blot: WiDr cell lysate stained with ARG55558 anti-ATP5B antibody.

  • ARG55558 anti-ATP5B antibody FACS image

    Flow Cytometry: WiDr cells stained with ARG55558 anti-ATP5B antibody (right histogram) or without primary antibody control (left histogram), followed by incubation with FITC labelled secondary antibody.

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