anti-ATP5C1 / ATPG antibody
Key features and details
- 产品描述:
- 反应物种:
- 预测物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
CAT.NO. : ARG58323
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Product Details
概述
| 产品描述 | Goat Polyclonal antibody recognizes ATP5C1 / ATPG |
|---|---|
| 反应物种 | Hu, Rat |
| 预测物种 | Ms, Cow, Dog |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ATP5C1 / ATPG |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide from the C-terminus of Human ATP5C1 / ATPG (NP_005165.1; NP_001001973.1). (C-TLTFNRTRQAVITKE) |
| 偶联标记 | Un-conjugated |
| 別名 | ATP5C1; ATPG; ATP5C; F-ATPase gamma subunit; ATP synthase subunit gamma, mitochondrial; ATP5CL1 |
应用说明
| 应用建议 |
| ||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 实际分子量 | ~ 33 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Ammonium sulphate precipitation followed by affinity purification with immunogen. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P35435 Rat ATP synthase subunit gamma, mitochondrial Swiss-port # P36542 Human ATP synthase subunit gamma, mitochondrial |
|---|---|
| 基因名称 | ATP5C1 |
| 全名 | ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1 |
| 背景介绍 | This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the gamma subunit of the catalytic core. Alternatively spliced transcript variants encoding different isoforms have been identified. This gene also has a pseudogene on chromosome 14. [provided by RefSeq, Jul 2008] |
| 生物功能 | Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and the central stalk which is part of the complex rotary element. The gamma subunit protrudes into the catalytic domain formed of alpha(3)beta(3). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. [UniProt] |
| 预测分子量 | 33 kDa |
检测图片 (2)
ARG58323 anti-ATP5C1 / ATPG antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human heart stained with ARG58323 anti-ATP5C1 / ATPG antibody at 5 µg/ml dilution. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
ARG58323 anti-ATP5C1 / ATPG antibody WB image
Western blot: 35µg of Rat heart lysate (in RIPA buffer) stained with ARG58323 anti-ATP5C1 / ATPG antibody at 0.01 µg/ml dilution. Primary incubation was 1 hour. Detected by chemiluminescence.
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