anti-ATP5H antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes ATP5H
  • 反应物种: Hu, Ms, Rat
  • 应用: ICC/IF, IHC-P, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: ATP5H
  • 抗原物种: Human
  • 抗原: Human ATP5H recombinant protein (Position: A2-L161). Human ATP5H shares 81% and 78% amino acid (aa) sequence identity with Mouse and Rat ATP5H, respectively.
  • Brand:
CAT.NO. : ARG58311
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes ATP5H
反应物种Hu, Ms, Rat
应用ICC/IF, IHC-P, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称ATP5H
抗原物种Human
抗原Human ATP5H recombinant protein (Position: A2-L161). Human ATP5H shares 81% and 78% amino acid (aa) sequence identity with Mouse and Rat ATP5H, respectively.
偶联标记Un-conjugated
別名ATPQ; ATP synthase subunit d, mitochondrial; ATPase subunit d
应用说明
应用建议
应用推荐稀释比
ICC/IF1:200 - 1:1000
IHC-P0.5 - 1 µg/ml
WB0.1 - 0.5 µg/ml
应用说明IHC-P: Antigen Retrieval: By heat mediation.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Affinity purification with immunogen.
缓冲液0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA.
抗菌剂0.05% Sodium azide
稳定剂5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 10476 Human ATP5H

GeneID: 641434 Rat ATP5H

GeneID: 71679 Mouse ATP5H

基因名称ATP5H
全名ATP synthase, H+ transporting, mitochondrial Fo complex, subunit d
背景介绍Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15. [provided by RefSeq, Jun 2010]
生物功能Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. [UniProt]
细胞定位Mitochondrion. Mitochondrion inner membrane. [UniProt]
预测分子量18 kDa
检测图片 (6)
  • ARG58311 anti-ATP5H antibody ICC image

    Immunocytochemistry: A549 cells stained with ARG58311 anti-ATP5H antibody.

  • ARG58311 anti-ATP5H antibody ICC/IF image

    Immunofluorescence: MCF-7 cells were blocked with 10% goat serum and then stained with ARG58311 anti-ATP5H antibody (green) at 5 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.

  • ARG58311 anti-ATP5H antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse intestine stained with ARG58311 anti-ATP5H antibody.

  • ARG58311 anti-ATP5H antibody WB image

    Western blot: 50 µg of Rat brain, 50 µg of Mouse brain, 50 µg of Human placenta and 40 µg of HeLa whole cell lysate stained with ARG58311 anti-ATP5H antibody at 0.5 µg/ml dilution.

  • ARG58311 anti-ATP5H antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat intestine stained with ARG58311 anti-ATP5H antibody.

  • ARG58311 anti-ATP5H antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human mammary cancer stained with ARG58311 anti-ATP5H antibody.

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