anti-B Raf antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原:
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Brand:
CAT.NO. : ARG54673
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes B Raf |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ELISA, ICC/IF, IHC, WB |
| 特异性 | At least two isoforms of B-raf are known to exist; this antibody will detect both isoforms. This antibody will not cross-react with C-raf. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | B Raf |
| 抗原 | Synthetic peptide (19 aa) within aa. 330-380 Human B-raf protein. |
| 偶联标记 | Un-conjugated |
| 別名 | BRAF1; v-Raf murine sarcoma viral oncogene homolog B1; Proto-oncogene B-Raf; p94; Serine/threonine-protein kinase B-raf; RAFB1; EC 2.7.11.1; B-RAF1; NS7 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | Human Brain Tissue Lysate | ||||||||||
| 实际分子量 | 99 kDa. Multiple post-translational modifications, commonly observed at 95-100 kDa. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P15056 Human Serine/threonine-protein kinase B-raf Swiss-port # P28028 Mouse Serine/threonine-protein kinase B-raf |
|---|---|
| 基因名称 | BRAF |
| 全名 | B-Raf proto-oncogene, serine/threonine kinase |
| 背景介绍 | B-raf Antibody: B-raf belongs to the raf/mil family of serine/threonine protein kinases and plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. The Ras/Raf/MEK/ERK and Ras/PI3K/PTEN/Akt pathways interact with each other to regulate growth and in some cases tumorigenesis. Mutations in B-raf have been associated with several cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung, leading to speculation on the possibility of B-raf as a therapeutic target for treating cancers. Mutations in this gene have also been associated with cardiofaciocutaneous syndrome (CFCS), a disease characterized by heart defects, mental retardation and a distinctive facial appearance. | |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Signaling Transduction antibody |
| 预测分子量 | 84 kDa |
| 翻译后修饰 | Phosphorylation at Ser-365 by SGK1 inhibits its activity. Methylation at Arg-671 decreases stability and kinase activity. Ubiquitinated by RNF149; which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF. |
检测图片 (3)
ARG54673 anti-B Raf antibody ICC/IF image
Immunofluorescence: Human small intestine tissue stained with ARG54673 anti-B Raf antibody at 20 μg/ml.
ARG54673 anti-B Raf antibody IHC image
Immunohistochemistry: Human small intestine tissue stained with ARG54673 anti-B Raf antibody at 2.5 μg/ml.
ARG54673 anti-B Raf antibody WB image
Western blot: Human brain tissue lysate stained with ARG54673 anti-B Raf antibody at (A) 1 and (B) 2 μg/ml.
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