anti-Bak antibody [38E2]
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Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
Product Details
| 产品描述 | Mouse Monoclonal antibody [38E2] recognizes Bak |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 38E2 |
| 同位型 | IgG2a, kappa |
| 靶点名称 | Bak |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 29-187 of Human Bak |
| 偶联标记 | Un-conjugated |
| 別名 | Apoptosis regulator BAK; BCL2L7; Bcl-2-like protein 7; CDN1; Bcl2-L-7; Bcl-2 homologous antagonist/killer; BAK-LIKE; BAK |
| 应用建议 |
| ||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | Swiss-port # O08734 Mouse Bcl-2 homologous antagonist/killer Swiss-port # Q16611 Human Bcl-2 homologous antagonist/killer |
|---|---|
| 基因名称 | BAK1 |
| 全名 | BCL2-antagonist/killer 1 |
| 背景介绍 | The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. [provided by RefSeq, Jul 2008] |
| 生物功能 | In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti-apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis. [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody; Cell Death antibody; Pro-apoptotic Bcl2 protein antibody |
| 预测分子量 | 23 kDa |
ARG57102 anti-Bak antibody [8B4] ICC/IF image
Immunofluorescence: HeLa cells line stained with ARG57102 anti-Bak antibody [8B4] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
ARG57102 anti-Bak antibody [8B4] FACS image
Flow Cytometry: HeLa cell line stained with ARG57102 anti-Bak antibody [8B4] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody: Mouse IgG (black line).
ARG57102 anti-Bak antibody [8B4] WB image
Western blot: 40 µg of 1) 293T, 2) HeLa, 3) A431, 4) A549, 5) Jurkat, 6) MCF7, and 7) PC3 cell lysates stained with ARG57102 anti-Bak antibody [8B4] at 1:1000.
ARG57102 anti-Bak antibody [8B4] ICC/IF image
Immunofluorescence: HeLa cells stained with ARG57102 anti-Bak antibody [8B4] at 1:500 (Red).
Hoechst 3342 (Blue) for nucleus staining.
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