anti-Bcl 7A antibody
Key features and details
- 产品描述:
- 反应物种:
- 预测物种:
- 应用:
- 特异性:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
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Brand:
Product Details
| 产品描述 | Goat Polyclonal antibody recognizes Bcl 7A |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Cow, Dog, Pig |
| 应用 | FACS, ICC/IF, WB |
| 特异性 | This antibody is expected to recognize both reported isoforms (NP_066273.1; NP_001019979.1). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Bcl 7A |
| 抗原物种 | Human |
| 抗原 | C-MKLEASQQNSEEM |
| 偶联标记 | Un-conjugated |
| 別名 | B-cell CLL/lymphoma 7 protein family member A; BCL7 |
| 应用建议 |
| ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | Swiss-port # Q4VC05 Human B-cell CLL/lymphoma 7 protein family member A |
|---|---|
| 背景介绍 | This gene is directly involved, with Myc and IgH, in a three-way gene translocation in a Burkitt lymphoma cell line. As a result of the gene translocation, the N-terminal region of the gene product is disrupted, which is thought to be related to the pathogenesis of a subset of high-grade B cell non-Hodgkin lymphoma. The N-terminal segment involved in the translocation includes the region that shares a strong sequence similarity with those of BCL7B and BCL7C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody |
| 预测分子量 | 23 kDa |
ARG63145 anti-Bcl 7A antibody WB image
Western blot: Human Lymphnode lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63145 anti-Bcl 7A antibody at 1 µg/ml dilution.
ARG63145 anti-Bcl 7A antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed U2OS cells permeabilized with 0.15% Triton. Cells were stained with ARG63145 anti-Bcl 7A antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Phalloidin (red) for Actin filaments staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
ARG63145 anti-Bcl 7A antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed Daudi cells permeabilized with 0.5% Triton. Cells were stained with ARG63145 anti-Bcl 7A antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
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