anti-BERP / RNF22 antibody

Key features and details

  • 产品描述: Goat Polyclonal antibody recognizes BERP / RNF22
  • 反应物种: Hu, Ms, Rat
  • 预测物种: Cow, Dog
  • 应用: WB
  • 特异性: Both variants (NP_006449.2; NP_150594.2;) represent the same protein. This antibody is expected to recognise all three human isoforms of this protein.
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: BERP / RNF22
  • Brand:
CAT.NO. : ARG63290
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Product Details
概述
产品描述Goat Polyclonal antibody recognizes BERP / RNF22
反应物种Hu, Ms, Rat
预测物种Cow, Dog
应用WB
特异性Both variants (NP_006449.2; NP_150594.2;) represent the same protein. This antibody is expected to recognise all three human isoforms of this protein.
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称BERP / RNF22
抗原物种Human
抗原AKREDSPGPEVQP-C
偶联标记Un-conjugated
別名BERP; RING finger protein 97; RNF97; HAC1; Brain-expressed RING finger protein; Tripartite motif-containing protein 3; RING finger protein 22; RNF22
应用说明
应用建议
应用推荐稀释比
WB0.1 - 0.3 µg/ml
应用说明WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
抗菌剂0.02% Sodium azide
稳定剂0.5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 10612 Human TRIM3

GeneID: 55992 Mouse TRIM3

GeneID: 83616 Rat TRIM3

背景介绍The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also called the 'RING-B-box-coiled-coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to cytoplasmic filaments. It is similar to a rat protein which is a specific partner for the tail domain of myosin V, a class of myosins which are involved in the targeted transport of organelles. The rat protein can also interact with alpha-actinin-4. Thus it is suggested that this human protein may play a role in myosin V-mediated cargo transport. Alternatively spliced transcript variants encoding the same isoform have been identified. [provided by RefSeq, Jul 2008]
研究领域Cell Biology and Cellular Response antibody
预测分子量81 kDa
检测图片 (3)
  • ARG63290 anti-BERP / RNF22 antibody WB image

    Western Blot: Mouse Brain lysate (RIPA buffer, 30µg total protein per lane) stained with ARG63290 anti-BERP / RNF22 antibody at 0.05 µg/ml dilution.

  • ARG63290 anti-BERP / RNF22 antibody WB image

    Western Blot: 1). Mock transfection; 2) TRIM3 (RC211928) expressing plasmid transfected HEK293 cell lysate standed with ARG63290 anti-BERP / RNF22 antibody

  • ARG63290 anti-BERP / RNF22 antibody WB image

    Western blot: 35 µg of Human cerebellum (A) and Rat brain (B) lysates (in RIPA buffer) stained with ARG63290 anti-BERP / RNF22 antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.

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