anti-beta Actin antibody
Key features and details
- 产品描述:
- 反应物种:
- 预测物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
Product Details
| 产品描述 | Rabbit Polyclonal antibody recognizes beta Actin |
|---|---|
| 反应物种 | Hu, Ms, Rat, Rb, Sheep |
| 预测物种 | Chk, Mk, Xenopus |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | beta Actin |
| 抗原物种 | Human |
| 抗原 | Recombinant Protein of Human beta Actin (NP_001092.1). |
| 偶联标记 | Un-conjugated |
| 別名 | PS1TP5BP1; BRWS1; Actin, cytoplasmic 1; Beta-actin |
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
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| 基因名称 | ACTB |
| 全名 | actin, beta |
| 背景介绍 | Beta actin is one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome. [provided by RefSeq, Aug 2017] |
| 生物功能 | Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30258 Loading Controls for Whole Cell Lysate Antibody Panel ARG30323 Inflammation Antibody Panel Related products: beta Actin antibodies; beta Actin Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| 研究领域 | Controls and Markers antibody; Signaling Transduction antibody; Loading Control antibody; Cytochrome-C fractionation Study antibody; Inflammation Study antibody; Tag Internal Control antibody |
| 预测分子量 | 42 kDa |
| 翻译后修饰 | ISGylated. Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity). Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148). |
ARG65683 anti-beta Actin antibody WB image
Western blot: 20 µg of HeLa, Mouse brain and Rat brain lysates stained with ARG65683 anti-beta Actin antibody at 1:10000 dilution.
ARG65683 anti-beta Actin antibody WB image
Western blot: 30 µg of 293T lysate stained with ARG65683 anti-beta Actin antibody at 1:3000 dilution.
ARG65683 anti-beta Actin antibody WB image
Western blot: 30 µg of 1) Rat brain, and 2) Mouse liver lysate stained with ARG65683 anti-beta Actin antibody at 1:3000 dilution.
ARG65683 anti-beta Actin antibody IHC-P image
Immunohistochemistry: Human ovary tissue stained with ARG65683 anti-beta Actin antibody at 1:200 dilution.
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