anti-beta Catenin phospho (Ser33) antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes beta Catenin phospho (Ser33)
  • 反应物种: Hu, Ms, Rat
  • 应用: IHC-P, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: beta Catenin
  • 抗原物种: Human
  • 抗原: Peptide sequence around phosphorylation site of serine 33 (L-D-S(p)-G-I) derived from Human β-Catenin.
  • Brand:
CAT.NO. : ARG51709
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes beta Catenin phospho (Ser33)
反应物种Hu, Ms, Rat
应用IHC-P, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称beta Catenin
抗原物种Human
抗原Peptide sequence around phosphorylation site of serine 33 (L-D-S(p)-G-I) derived from Human β-Catenin.
偶联标记Un-conjugated
別名CTNNB; armadillo; MRD19; Catenin beta-1; Beta-catenin
应用说明
应用建议
应用推荐稀释比
IHC-P1:50 - 1:100
WB1:500 - 1:1000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
缓冲液PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
抗菌剂0.02% Sodium azide
稳定剂50% Glycerol
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 12387 Mouse CTNNB1

GeneID: 1499 Human CTNNB1

GeneID: 84353 Rat CTNNB1

基因名称CTNNB1
全名catenin (cadherin-associated protein), beta 1, 88kDa
背景介绍Involved in the regulation of cell adhesion and in signal transduction through the Wnt pathway.
生物功能Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22647378, PubMed:22699938, PubMed:22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity). [UniProt]
产品亮点Related Antibody Duos and Panels:
ARG30144 Phospho beta Catenin Antibody Panel (Total, pS33, pS37, pT41/pS45)
Related products:
beta Catenin antibodies; beta Catenin Duos / Panels; Anti-Rabbit IgG secondary antibodies;
研究领域Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody
预测分子量85 kDa
翻译后修饰Phosphorylation at Ser-552 by AMPK promotes stabilizion of the protein, enhancing TCF/LEF-mediated transcription (By similarity). Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33. Phosphorylated by NEK2. EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding. Phosphorylated on Ser-33 and Ser-37 by HIPK2 and GSK3B, this phosphorylation triggers proteasomal degradation (PubMed:25169422). Phosphorylation on Ser-191 and Ser-246 by CDK5. Phosphorylation by CDK2 regulates insulin internalization. Phosphorylation by PTK6 at Tyr-64, Tyr-142, Tyr-331 and/or Tyr-333 with the predominant site at Tyr-64 is not essential for inhibition of transcriptional activity.
Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation (By similarity).
S-nitrosylation at Cys-619 within adherens junctions promotes VEGF-induced, NO-dependent endothelial cell permeability by disrupting interaction with E-cadherin, thus mediating disassembly adherens junctions.
O-glycosylation at Ser-23 decreases nuclear localization and transcriptional activity, and increases localization to the plasma membrane and interaction with E-cadherin CDH1.
Deacetylated at Lys-49 by SIRT1.
检测图片 (2)
  • ARG51709 anti-beta Catenin phospho (Ser33) antibody WB image

    Western blot: Extracts from MCF-7 cells untreated or treated with Calyculin A stained with ARG51709 anti-beta Catenin phospho (Ser33) antibody.

  • ARG51709 anti-beta Catenin phospho (Ser33) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51709 anti-beta Catenin phospho (Ser33) antibody (left) or the same antibody preincubated with blocking peptide (right).

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