anti-beta II Tubulin antibody [5B3]
![anti-beta II Tubulin antibody [5B3]](/upload/image/products/ARG57104_WB_1_210_205.jpg)
![anti-beta II Tubulin antibody [5B3]](/upload/image/products/ARG57104_IF_1.jpg)
![anti-beta II Tubulin antibody [5B3]](/upload/image/products/ARG57104_IHC_1.jpg)
![anti-beta II Tubulin antibody [5B3]](/upload/image/products/ARG57104_WB_1.jpg)
Key features and details
- 产品描述:
- 反应物种:
- 应用:
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- 克隆:
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- 同位型:
- 靶点名称:
- 抗原物种:
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CAT.NO. : ARG57104
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Product Details
概述
| 产品描述 | Mouse Monoclonal antibody [5B3] recognizes beta II Tubulin |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 5B3 |
| 同位型 | IgG2a, kappa |
| 靶点名称 | beta II Tubulin |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-445 of Human beta II Tubulin |
| 偶联标记 | Un-conjugated |
| 別名 | Tubulin beta-2B chain; bA506K6.1; PMGYSA |
应用说明
| 应用建议 |
| ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 0.1M Sodium citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TUBB2B |
| 全名 | tubulin, beta 2B class IIb |
| 背景介绍 | The protein encoded by this gene is a beta isoform of tubulin, which binds GTP and is a major component of microtubules. This gene is highly similar to TUBB2A and TUBB2C. Defects in this gene are a cause of asymmetric polymicrogyria. [provided by RefSeq, Mar 2010] |
| 生物功能 | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity). TUBB2B is implicated in neuronal migration. [UniProt] |
| 预测分子量 | 50 kDa |
| 翻译后修饰 | Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866). Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable). Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules. |
检测图片 (3)
ARG57104 anti-beta II Tubulin antibody [5B3] ICC/IF image
Immunofluorescence: SW480 cells stained with ARG57104 anti-beta II Tubulin antibody [5B3] at 1:250 (Red). Arrow head: Mitotic cell, Arrow: MTOC (microtubule organizing center).
ARG57104 anti-beta II Tubulin antibody [5B3] IHC-P image
Immunohistochemistry: Paraffin-embedded sections of colorectal cancer tissue stained with ARG57104 anti-beta II Tubulin antibody [5B3] at 1:50 for 2 hours at RT. Antigen Retrieval: Boil tissue section in 0.1M Sodium citrate buffer (pH 6.0) for 20 min.
ARG57104 anti-beta II Tubulin antibody [5B3] WB image
Western blot: 35 µg of HeLa, 293T and Mouse brain lysates stained with ARG57104 anti-beta II Tubulin antibody [5B3] at 1:500.
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