anti-Bid antibody [4D3]

CAT.NO. : ARG56970

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Size:

50 μl

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

概述

产品描述	Mouse Monoclonal antibody [4D3] recognizes Bid
反应物种	Hu
应用	ICC/IF, WB
宿主	Mouse
克隆	Monoclonal
克隆号	4D3
同位型	IgG1, kappa
靶点名称	Bid
抗原物种	Human
抗原	Recombinant fragment around aa. 1-195 of Human Bid.
偶联标记	Un-conjugated
別名	p22 BID; BID; p15 BID; p11 BID; BH3-interacting domain death agonist; p13 BID; FP497

应用说明

应用建议

应用	推荐稀释比
ICC/IF	Assay-dependent
WB	1:1000 - 1:2000

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein G.
缓冲液	PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	10% Glycerol
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 637 Human BID Swiss-port # P55957 Human BH3-interacting domain death agonist
基因名称	BID
全名	BH3 interacting domain death agonist
背景介绍	This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]
生物功能	The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2. [UniProt]
研究领域	Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Apoptosis Marker antibody; Pro-apoptotic Bcl2 protein antibody
预测分子量	22 kDa
翻译后修饰	TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products. p15 BID is ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation.

检测图片 (2)

ARG56970 anti-Bid antibody [4D3] ICC/IF image
Immunoflorescense: HeLa cell line stained with ARG56970 anti-Bid antibody [4D3] at 1:100 (green).
DAPI (Blue) for nucleus staining.
ARG56970 anti-Bid antibody [4D3] WB image
Western blot: 20 µg of Jurkat stained with ARG56970 anti-Bid antibody [4D3] at 1:1000.

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