anti-BiP / GRP78 antibody

Key features and details

  • 产品描述: Mouse Monoclonal antibody recognizes BiP / GRP78
  • 反应物种: Hu, Rat
  • 应用: ICC/IF, WB
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 同位型: IgG
  • 靶点名称: BiP / GRP78
  • 抗原物种: Human
  • 抗原: Purified recombinant human BiP/GRP78 protein fragments expressed in E.coli.
  • Brand:
CAT.NO. : ARG54027
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Product Details
概述
产品描述Mouse Monoclonal antibody recognizes BiP / GRP78
反应物种Hu, Rat
应用ICC/IF, WB
宿主Mouse
克隆Monoclonal
同位型IgG
靶点名称BiP / GRP78
抗原物种Human
抗原Purified recombinant human BiP/GRP78 protein fragments expressed in E.coli.
偶联标记Un-conjugated
別名Heat shock 70 kDa protein 5; BiP; GRP-78; Endoplasmic reticulum lumenal Ca; MIF2; 78 kDa glucose-regulated protein; GRP78; Immunoglobulin heavy chain-binding protein; HEL-S-89n; BIP; 2+
应用说明
应用建议
应用推荐稀释比
ICC/IF1:50
WB1:1000
应用说明IHC-P: Antigen Retrieval: Pressure cooking in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Affinity purified
缓冲液PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol
抗菌剂0.02% Sodium azide
稳定剂50% Glycerol
浓度0.8 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 25617 Rat HSPA5

GeneID: 3309 Human HSPA5

Swiss-port # P06761 Rat 78 kDa glucose-regulated protein

Swiss-port # P11021 Human 78 kDa glucose-regulated protein

基因名称HSPA5
全名heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
背景介绍Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER
生物功能Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate. [UniProt]
细胞定位Endoplasmic reticulum lumen. Melanosome
研究领域Controls and Markers antibody; ER Marker antibody; Endoplasmic reticulum antibody
预测分子量72 kDa
检测图片 (2)
  • ARG54027 anti-BiP / GRP78 antibody WB image

    Western blot: 30 µg of HeLa cell lysate stained with ARG54027 anti-BiP / GRP78 antibody at 1:1000 dilution.

  • ARG54027 anti-BiP / GRP78 antibody ICC/IF image

    Immunofluorescence: HeLa cells fixed with 4% Paraformaldehyde and stained with ARG54027 anti-BiP / GRP78 antibody at 1:50 dilution.

参考文献

Endoplasmic reticulum stress promotes sorafenib resistance via miR-188-5p/hnRNPA2B1-mediated upregulation of PKM2 in hepatocellular carcinoma

WB / Human

Bei Zhou et al.
Mol Ther Nucleic Acids.,  (2021)

publication_link

 

hr_line

Study of isolation and characterization of extracellular vesicles in senescent tumor cells induced by chemotherapeutics.

WB / Human

Qi Zhou et al.
Chinese Journal of Immunology.,  (2020)

publication_link

 

hr_line

Melatonin Increases the Sensitivity of Hepatocellular Carcinoma to Sorafenib through the PERK-ATF4-Beclin1 Pathway

WB, ICC/IF / Human

Zhou Bei et al.
Int J Biol Sci.,  (2019)

publication_link

 

hr_line

Effects of calpain inhibitor on the apoptosis of hepatic stellate cells induced by calcium ionophore A23187.

WB / Human

Li Yanan et al.
J Cell Biochem.,  (2018)

publication_link

 

hr_line

Effects of calcium Ionophore A23187 on the apoptosis of hepatic stellate cells stimulated by transforming growth factor-β1.

WB / Rat

Li Yanan et al.
Cell Mol Biol Lett.,  (2018)

publication_link

 

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