anti-BNP antibody [50E1]
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Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
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Brand:
Product Details
| 产品描述 | Mouse Monoclonal antibody [50E1] recognizes BNP |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA, WB |
| 特异性 | Human BNP and proBNP |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 50E1 |
| 同位型 | IgG1 |
| 靶点名称 | BNP |
| 抗原物种 | Human |
| 抗原 | Human synthetic BNP (whole molecule) |
| 抗原表位 | CKVLRRH (a.a. 26-32 of human BNP) |
| 偶联标记 | Un-conjugated |
| 別名 | 4-27; Natriuretic peptides B; 1-28; 1-29; 5-32; BNP; 1-32; 1-30; BNP-32; 5-29; 2-31; 4-30; 4-31; 4-32; 3-29; Gamma-brain natriuretic peptide; 3-30; 5-31; 3-32; 4-29 |
| 应用建议 |
| ||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | sELISA: Recommended antibody pairs in sandwich immunoassay for BNP (capture - detection): ARG10146 - ARG10609 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Protein A affinity purified. |
| 缓冲液 | PBS (pH 7.4) and 0.1% Sodium azide |
| 抗菌剂 | 0.1% Sodium azide |
| 浓度 | 1.0-2.0 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
|---|---|
| 基因名称 | NPPB |
| 全名 | natriuretic peptide B |
| 背景介绍 | This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Mutations in this gene have been associated with postmenopausal osteoporosis. [provided by RefSeq, Nov 2014] |
| 生物功能 | Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30278 BNP ELISA Antibody Duo Related products: BNP antibodies; BNP ELISA Kits; BNP Duos / Panels; Anti-Mouse IgG secondary antibodies; |
| 研究领域 | Cell Biology and Cellular Response antibody; Signaling Transduction antibody |
| 预测分子量 | 15 kDa |
| 翻译后修饰 | The brain natriuretic peptide 32 form is cleaved at Pro-104 by the prolyl endopeptidase FAP (seprase) activity (in vitro). |
ARG10146 anti-BNP antibody [50E1] WB image
Western blot: Human synthetic BNP stained with ARG10146 anti-BNP antibody [50E1].
ARG10146 anti-BNP antibody [50E1] FLISA image
FLISA: Biotinylated ARG10146 anti-BNP antibody [50E1] used as capture antibody and Eu3+ - labeled ARG10609 anti-BNP antibody [24C5] used as detection antibody. Mixture of antibodies (50 µl) and synthetic BNP (50 µl) is incubated for 30 min at RT in streptavidincoated plate.
ARG10146 anti-BNP antibody [50E1] stability studies image
Stability studies: Recombinant glycosylated proBNP (from mammalian cell line) (green) and endogenous proBNP, purified from pooled HF patients plasma (red) were reconstituted in pooled normal human EDTA-plasma and incubated at RT for different time periods.
Immunological activity was measured by immunoassay, utilizing ARG10146 anti-BNP antibody [50E1] as capture and ARG10609 anti-BNP antibody [24C5] as detection.ARG10146 anti-BNP antibody [50E1] WB image
Western blot: Recombinant proBNP (E. coli expressed) stained with ARG10146 anti-BNP antibody [50E1].
克隆号文献
Secretion of glycosylated pro-B-type natriuretic peptide from normal cardiomyocytes.
WB, ICC/IF / Human
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