anti-BubR1 antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes BubR1
  • 反应物种: Hu
  • 应用: FACS, IHC-P, IP, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: BubR1
  • 抗原物种: Human
  • 抗原: Synthetic peptide derived from Human BubR1.
  • Brand:
CAT.NO. : ARG41381
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes BubR1
反应物种Hu
应用FACS, IHC-P, IP, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称BubR1
抗原物种Human
抗原Synthetic peptide derived from Human BubR1.
偶联标记Un-conjugated
別名MVA1; Bub1A; Mitotic checkpoint serine/threonine-protein kinase BUB1 beta; MAD3/BUB1-related protein kinase; SSK1; Mitotic checkpoint kinase MAD3L; Protein SSK1; hBUBR1; EC 2.7.11.1; MAD3L; BUBR1; BUB1beta
应用说明
应用建议
应用推荐稀释比
FACS1:50
IHC-P1:50 - 1:200
IP1:30
WB1:1000 - 1:5000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量~ 120 kDa
属性
形式Liquid
纯化Affinity purified.
缓冲液PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
抗菌剂0.02% Sodium azide
稳定剂50% Glycerol
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 701 Human BUB1B

Swiss-port # O60566 Human Mitotic checkpoint serine/threonine-protein kinase BUB1 beta

基因名称BUB1B
全名BUB1 mitotic checkpoint serine/threonine kinase B
背景介绍This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer. [provided by RefSeq, Jul 2008]
生物功能Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression. [UniProt]
细胞定位Cytoplasm. Nucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Cytoplasmic in interphase cells. Associates with the kinetochores in early prophase. Kinetochore localization requires BUB1, PLK1 and KNL1. [UniProt]
预测分子量120 kDa
翻译后修饰Proteolytically cleaved by caspase-3 in a cell cycle specific manner. The cleavage might be involved in the durability of the cell cycle delay. Caspase-3 cleavage is associated with abrogation of the mitotic checkpoint. The major site of cleavage is at Asp-610.

Acetylation at Lys-250 regulates its degradation and timing in anaphase entry.

Ubiquitinated. Degraded by the proteasome.

Sumoylated with SUMO2 and SUMO3. The sumoylation mediates the association with CENPE at the kinetochore.

Autophosphorylated in vitro. Intramolecular autophosphorylation is stimulated by CENPE. Phosphorylated during mitosis and hyperphosphorylated in mitotically arrested cells. Phosphorylation at Ser-670 and Ser-1043 occurs at kinetochores upon mitotic entry with dephosphorylation at the onset of anaphase. [UniProt]
检测图片 (1)
  • ARG41381 anti-BubR1 antibody WB image

    Western blot: HepG2 cell lysate stained with ARG41381 anti-BubR1 antibody.

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