anti-c-Myb antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
-
Brand:
Product Details
| 产品描述 | Rabbit Polyclonal antibody recognizes c-Myb |
|---|---|
| 反应物种 | Hu, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | c-Myb |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of Human c-Myb. |
| 偶联标记 | Un-conjugated |
| 別名 | Transcriptional activator Myb; Proto-oncogene c-Myb; c-myb_CDS; c-myb; Cmyb; efg |
| 应用建议 |
| ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | ~ 72 kDa |
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 30% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
|---|---|
| 基因名称 | MYB |
| 全名 | v-myb avian myeloblastosis viral oncogene homolog |
| 背景介绍 | This gene encodes a transcription factor that is a member of the MYB family of transcription factor genes. The protein contains three domains, an N-terminal DNA-binding domain, a central transcriptional activation domain and a C-terminal domain involved in transcriptional repression. This protein plays an essential role in the regulation of hematopoiesis and may play a role in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2009] |
| 生物功能 | Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 72 kDa |
| 翻译后修饰 | Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation. Phosphorylated by NLK on multiple sites, which induces proteasomal degradation. Phosphorylated by HIPK1. This phosphorylation reduces MYB transcription factor activity but not MYB protein levels. [UniProt] |
ARG66551 anti-c-Myb antibody ICC/IF image
Immunofluorescence: Formalin-fixed HeLa cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were stained with ARG66551 anti-c-Myb antibody (red) in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. DAPI was used to stain the cell nuclei (blue).
ARG66551 anti-c-Myb antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human tonsil tissue. Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). The section was then stained with ARG66551 anti-c-Myb antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
ARG66551 anti-c-Myb antibody WB image
Western blot: K562 and Rat skeletal muscle lysates stained with ARG66551 anti-c-Myb antibody.
New Products
