anti-Calbindin antibody

Key features and details

  • 产品描述: Goat Polyclonal antibody recognizes Calbindin
  • 反应物种: Hu, Ms, Rat
  • 预测物种: Cow, Dog, Pig
  • 应用: IHC-Fr, IHC-P, WB
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: Calbindin
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG40287
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Product Details
概述
产品描述Goat Polyclonal antibody recognizes Calbindin
反应物种Hu, Ms, Rat
预测物种Cow, Dog, Pig
应用IHC-Fr, IHC-P, WB
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称Calbindin
抗原物种Human
抗原Synthetic peptide around the internal region of Human Calbindin. (NP_004920.1. C-KTFVDQYGQRDDGK)
偶联标记Un-conjugated
別名Vitamin D-dependent calcium-binding protein, avian-type; Calbindin; CALB; Calbindin D28; D-28K
应用说明
应用建议
应用推荐稀释比
IHC-Fr0.1 - 0.3 µg/ml
IHC-P5 µg/ml
WB0.03 - 0.1 µg/ml
应用说明WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0) at 80°C for 30 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量~ 26 kDa
属性
形式Liquid
纯化Ammonium sulphate precipitation followed by affinity purification with immunogen.
缓冲液Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂0.02% Sodium azide
稳定剂0.5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 12307 Mouse CALB1

GeneID: 793 Human CALB1

GeneID: 83839 Rat CALB1

基因名称CALB1
全名calbindin 1, 28kDa
背景介绍The protein encoded by this gene is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease. [provided by RefSeq, Jan 2015]
生物功能Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. [UniProt]
预测分子量30 kDa
检测图片 (4)
  • ARG40287 anti-Calbindin antibody IHC-Fr image

    Immunohistochemistry: PFA-perfused cryosection of Human hypothalamus tissue stained with ARG40287 anti-Calbindin antibody at 0.1 µg/ml dilution.

  • ARG40287 anti-Calbindin antibody WB image

    Western blot: 35 µg of Human cerebellum lysate (in RIPA buffer) stained with ARG40287 anti-Calbindin antibody at 0.03 µg/ml dilution and incubated at RT for 1 hour.

  • ARG40287 anti-Calbindin antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human kidney stained with ARG40287 anti-Calbindin antibody at 5 µg/ml dilution. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).

  • ARG40287 anti-Calbindin antibody WB image

    Western blot: 35 µg of Mouse brain and Rat brain lysates (in RIPA buffer) stained with ARG40287 anti-Calbindin antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.

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