anti-Calpain 1 antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
CAT.NO. : ARG40288
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Product Details
概述
| 产品描述 | Goat Polyclonal antibody recognizes Calpain 1 |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Calpain 1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the internal region of Human Calpain 1. (NP_005177.2. C-SEKSAGTVELDD) |
| 偶联标记 | Un-conjugated |
| 別名 | EC 3.4.22.52; CANP1; Calpain-1 large subunit; Calpain-1 catalytic subunit; Calpain mu-type; muCANP; Cell proliferation-inducing gene 30 protein; Micromolar-calpain; CANP 1; CANP; muCL; CANPL1; Calcium-activated neutral proteinase 1 |
应用说明
| 应用建议 |
| ||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 实际分子量 | ~ 80 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Ammonium sulphate precipitation followed by affinity purification with immunogen. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CAPN1 |
| 全名 | calpain 1, (mu/I) large subunit |
| 背景介绍 | The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010] |
| 生物功能 | Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. [UniProt] |
| 细胞定位 | Cytoplasm. Cell membrane. Note=Translocates to the plasma membrane upon Ca(2+) binding. In granular keratinocytes and in lower corneocytes, colocalizes with FLG and FLG2 (PubMed:21531719). [UniProt] |
| 预测分子量 | 82 kDa |
| 翻译后修饰 | Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms. [UniProt] |
检测图片 (2)
ARG40288 anti-Calpain 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human kidney tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG40288 anti-Calpain 1 antibody at 5 µg/ml dilution followed by AP-staining.
ARG40288 anti-Calpain 1 antibody WB image
Western blot: 35 µg of Human spleen lysate (in RIPA buffer) stained with ARG40288 anti-Calpain 1 antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
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