anti-Cas9 (S. aureus) antibody

Key features and details

  • 产品描述: Chicken Polyclonal antibody recognizes Cas9 (S. aureus)
  • 反应物种: Bacteria
  • 应用: ICC/IF, IHC-Fr, WB
  • 宿主: Chicken
  • 克隆: Polyclonal
  • 同位型: IgY
  • 靶点名称: Cas9 (S. aureus)
  • 抗原物种: Bacteria
  • 抗原: C-terminal region of S. aureus Cas9, aa. 803-1053 of sequence CCK74173, expressed in and purified from E. coli.
  • Brand:
CAT.NO. : ARG10681
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Product Details
概述
产品描述Chicken Polyclonal antibody recognizes Cas9 (S. aureus)
反应物种Bacteria
应用ICC/IF, IHC-Fr, WB
宿主Chicken
克隆Polyclonal
同位型IgY
靶点名称Cas9 (S. aureus)
抗原物种Bacteria
抗原C-terminal region of S. aureus Cas9, aa. 803-1053 of sequence CCK74173, expressed in and purified from E. coli.
偶联标记Un-conjugated
应用说明
应用建议
应用推荐稀释比
ICC/IF1:1000 - 1:5000
IHC-Fr1:1000 - 1:5000
WB1:1000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
缓冲液PBS and 0.02% Sodium azide.
抗菌剂0.02% Sodium azide
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
检测图片 (2)
  • ARG10681 anti-Cas9 (S. aureus) antibody ICC/IF image

    Immunocytochemistry: Transfected HEK293 cells which overexpressing GFP-Cas9 (SA) fusion protein were stained with ARG10681 anti-Cas9 (S. aureus) antibody. Cells which are transfected with GFP-Cas9 are bright green (left panel). Staining with ARG10681 anti-Cas9 (S. aureus) antibody is shown in red in middle panel. In merged image (right panel), most Hek293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Red antibody staining is only seen in cells which express GFP, as expected, and the superimposition of green and red results in an orange signal.

  • ARG10681 anti-Cas9 (S. aureus) antibody WB image

    Western blot: 1) HEK293 cells overexpressing a fusion protein containing GFP and the C-terminus of Cas9 from S. aureus, and 2) Non-transfected HEK293 cells stained with ARG10681 anti-Cas9 (S. aureus) antibody at 1:1000 dilution.

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