anti-Casein Kinase 1 delta antibody
CAT.NO. : ARG63958
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概述
产品描述 | Goat Polyclonal antibody recognizes Casein Kinase 1 delta |
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反应物种 | Hu |
预测物种 | Ms, Rat, Dog |
应用 | ICC/IF, WB |
特异性 | This antibody is expected to recognise both reported isoforms (NP_001884.2 and NP_620693.1) |
宿主 | Goat |
克隆 | Polyclonal |
同位型 | IgG |
靶点名称 | Casein Kinase 1 delta |
抗原物种 | Human |
抗原 | C-DREERLRHSRNPATR |
偶联标记 | Un-conjugated |
別名 | ASPS; EC 2.7.11.26; CKIdelta; CKId; HCKID; Casein kinase I isoform delta; Tau-protein kinase CSNK1D; EC 2.7.11.1; CKI-delta; FASPS2 |
应用说明
应用建议 |
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应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
形式 | Liquid |
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纯化 | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
抗菌剂 | 0.02% Sodium azide |
稳定剂 | 0.5% BSA |
浓度 | 0.5 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
数据库连接 | |
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背景介绍 | This gene is a member of the casein kinase I (CKI) gene family whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is highly similar to the mouse and rat CK1 delta homologs. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
研究领域 | Cell Biology and Cellular Response antibody; Cell Death antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody |
预测分子量 | 47 kDa |
翻译后修饰 | Autophosphorylated on serine and threonine residues; this autophosphorylation represses activity. Reactivated by phosphatase-mediated dephosphorylation. May be dephosphorylated by PP1. |
检测图片 (2)
ARG63958 anti-Casein Kinase 1 delta antibody WB image
Western blot: 293 cell lysate (35 µg protein in RIPA buffer) stained with ARG63958 anti-Casein Kinase 1 delta antibody at 0.3 µg/ml dilution.
ARG63958 anti-Casein Kinase 1 delta antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed U251 cells permeabilized with 0.15% Triton. Cells were stained with ARG63958 anti-Casein Kinase 1 delta antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
