anti-Caspase 3 antibody [C33]

Key features and details

产品描述: Mouse Monoclonal antibody [C33] recognizes Caspase 3
反应物种: Hu
应用: WB
特异性: The antibody recognizes both proform and the cleaved large fragment of caspase-3 in samples of human, mouse and rat origins.
宿主: Mouse
克隆: Monoclonal
克隆号: C33
同位型: IgG1
靶点名称: Caspase 3

Brand:

CAT.NO. : ARG20001

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Size:

50 μg 25 μg

Trail, Bulk size or Custom requests Please contact us

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

概述

产品描述	Mouse Monoclonal antibody [C33] recognizes Caspase 3
反应物种	Hu
应用	WB
特异性	The antibody recognizes both proform and the cleaved large fragment of caspase-3 in samples of human, mouse and rat origins.
宿主	Mouse
克隆	Monoclonal
克隆号	C33
同位型	IgG1
靶点名称	Caspase 3
抗原物种	Human
抗原	Recombinant human Caspase-3
偶联标记	Un-conjugated
別名	CASP3; Caspase 3; Apopain; CPP32; CPP32B; Caspase 3, Apoptosis-Related Cysteine Peptidase; Caspase 3, Apoptosis-Related Cysteine Protease; SREBP Cleavage Activity 1; Cysteine Protease CPP32; Protein Yama; EC 3.4.22.56; Caspase-3; CASP-3; CPP-32; SCA-1; Yama; PARP Cleavage Protease; Procaspase3; EC 3.4.22

应用说明

应用建议

应用	推荐稀释比
WB	0.5 - 4 µg/ml

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Affinity Purified Antibody
缓冲液	PBS, 50% Glycerol, 0.5 mg/ml BSA and 0.01% Thimerosal
抗菌剂	0.01% Thimerosal
稳定剂	50% Glycerol, 0.5 mg/ml BSA
浓度	0.2 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 836 Human CASP3 Swiss-port # P42574 Human Caspase-3
基因名称	CASP3
全名	caspase 3, apoptosis-related cysteine peptidase
背景介绍	The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017]
生物功能	Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface. [UniProt]
细胞定位	Cytoplasm. [UniProt]
产品亮点	Related Antibody Duos and Panels: ARG30105 Apoptosis Marker Antibody Duo (Caspase3, PARP) ARG30110 Mitochondria/Caspase dependant Apoptosis Antibody Panel (Caspase3, Caspase9, Cytochrome c, PARP) (WB) Related products: Caspase 3 antibodies; Caspase 3 Duos / Panels; Anti-Mouse IgG secondary antibodies;
研究领域	Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Neuroscience antibody; Apoptosis Marker antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody
预测分子量	32 kDa
翻译后修饰	Acetylation, Phosphoprotein, S-nitrosylation, Zymogen. [UniProt]

检测图片 (2)

ARG20001 anti-Caspase 3 antibody [C33] WB image
Western blot: 30 µg of HeLa cells untreated or treated with Staurosporine (1μM, overnight). The blots were stained with ARG20001 anti-Caspase 3 antibody [C33] at 1:500 dilution.
ARG20001 anti-Caspase 3 antibody [C33] WB image
Western blot: 20 µg of HeLa cell lysates stained with ARG20001 anti-Caspase 3 antibody [C33] at 2 µg/ml dilution.

参考文献

Moonlighting glyceraldehyde-3-phosphate dehydrogenase of Lactobacillus gasseri inhibits keratinocyte apoptosis and skin inflammation in experimental atopic dermatitis

WB / Mouse

Pei-Chi Chen et al.
Asian Pac J Allergy Immunol., (2024)