anti-Caspase 3 (cleaved) antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes Caspase 3 (cleaved)
  • 反应物种: Hu, Ms, Rat
  • 应用: IHC-P, WB
  • 特异性: This antibody detects fragment of activated Caspase 3 p17 protein resulting from cleavage adjacent to D175.
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: Caspase 3 (cleaved)
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG66671
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes Caspase 3 (cleaved)
反应物种Hu, Ms, Rat
应用IHC-P, WB
特异性This antibody detects fragment of activated Caspase 3 p17 protein resulting from cleavage adjacent to D175.
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称Caspase 3 (cleaved)
抗原物种Human
抗原Synthetic peptide within aa. 126-175 of Human Caspase 3.
偶联标记Un-conjugated
別名CASP3; Caspase 3; Apopain; CPP32; CPP32B; Caspase 3, Apoptosis-Related Cysteine Peptidase; Caspase 3, Apoptosis-Related Cysteine Protease; SREBP Cleavage Activity 1; Cysteine Protease CPP32; Protein Yama; EC 3.4.22.56; Caspase-3; CASP-3; CPP-32; SCA-1; Yama; PARP Cleavage Protease; Procaspase3; EC 3.4.22
应用说明
应用建议
应用推荐稀释比
IHC-P1:50 - 1:300
WB1:500 - 1:2000
应用说明IHC-P: Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照293 and Mouse kidney
实际分子量17/19 kDa (cleaved)
属性
形式Liquid
纯化Affinity purification with immunogen.
缓冲液PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA.
抗菌剂0.02% Sodium azide
稳定剂50% Glycerol and 0.5% BSA
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 12367 Mouse CASP3

GeneID: 25402 Rat CASP3

GeneID: 836 Human CASP3

基因名称CASP3
全名caspase 3, apoptosis-related cysteine peptidase
背景介绍The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017]
生物功能Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface. [UniProt]
细胞定位Cytoplasm. [UniProt]
产品亮点Related Antibody Duos and Panels:
ARG30105 Apoptosis Marker Antibody Duo (Caspase3, PARP)
ARG30110 Mitochondria/Caspase dependant Apoptosis Antibody Panel (Caspase3, Caspase9, Cytochrome c, PARP) (WB)
Related products:
Caspase 3 antibodies; Caspase 3 Duos / Panels;
研究领域Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Neuroscience antibody; Apoptosis Marker antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody
预测分子量17 kDa (cleaved)
翻译后修饰Acetylation, Phosphoprotein, S-nitrosylation, Zymogen. [UniProt]
检测图片 (4)
  • ARG66671 anti-Caspase 3 (cleaved) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat testis tissue. Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0) for 20 min. Tissue section was stained with ARG66671 anti-Caspase 3 (cleaved) antibody at 1:200 dilution, overnight at 4°C. Negative control was used by secondary antibody only.

  • ARG66671 anti-Caspase 3 (cleaved) antibody WB image

    Western blot: 293 cell lysate stained with ARG66671 anti-Caspase 3 (cleaved) antibody.

  • ARG66671 anti-Caspase 3 (cleaved) antibody IHC image

    Immunohistochemistry: Mouse liver tissue stained with ARG66671 anti-Caspase 3 (cleaved) antibody (red) at 1:200 dilution, overnight at 4°C. DAPI (blue) for nuclear staining.

  • ARG66671 anti-Caspase 3 (cleaved) antibody WB image

    Western blot: Mouse kidney lysate stained with ARG66671 anti-Caspase 3 (cleaved) antibody at 1:1000 dilution, overnight at 4°C.

参考文献

Resveratrol improves ovarian state by inhibiting apoptosis of granulosa cells

WB / Human

Ying Liang et al.
Gynecol Endocrinol.,  (2023)

publication_link

 

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