anti-Caveolin 1 antibody

Key features and details

  • 产品描述: Goat Polyclonal antibody recognizes Caveolin 1
  • 反应物种: Hu
  • 预测物种: Ms, Rat, Dog, Pig
  • 应用: FACS, ICC/IF, IHC-P, WB
  • 特异性: No cross-reactivity expected to Caveolin 2 and Caveolin 3
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: Caveolin 1
  • Brand:
CAT.NO. : ARG63780
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Product Details
概述
产品描述Goat Polyclonal antibody recognizes Caveolin 1
反应物种Hu
预测物种Ms, Rat, Dog, Pig
应用FACS, ICC/IF, IHC-P, WB
特异性No cross-reactivity expected to Caveolin 2 and Caveolin 3
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称Caveolin 1
抗原物种Human
抗原C-DELSEKQVYDAH
偶联标记Un-conjugated
別名CGL3; LCCNS; PPH3; MSTP085; VIP21; BSCL3; Caveolin-1
应用说明
应用建议
应用推荐稀释比
FACS10 µg/ml
ICC/IF10 µg/ml
IHC-PAssay - dependent
WB0.001 - 0.01 µg/ml
应用说明WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
抗菌剂0.02% Sodium azide
稳定剂0.5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 857 Human CAV1

Swiss-port # Q03135 Human Caveolin-1

背景介绍The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade. Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Mutations in this gene have been associated with Berardinelli-Seip congenital lipodystrophy. Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1.[provided by RefSeq, Mar 2010]
研究领域Endosome Marker antibody
预测分子量20 kDa
翻译后修饰The initiator methionine for isoform 2 is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.
Phosphorylated at Tyr-14 by ABL1 in response to oxidative stress.
检测图片 (7)
  • ARG63780 anti-Caveolin 1 antibody WB image

    Western Blot: Human Spleen lysate (35 µg protein in RIPA buffer) stained with ARG63780 anti-Caveolin 1 antibody at 0.01 µg/ml dilution.

  • ARG63780 anti-Caveolin 1 antibody ICC/IF image

    Immunofluorescence: Paraformaldehyde fixed A431 cells permeabilized with 0.15% Triton. Cells were stained with ARG63780 anti-Caveolin 1 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.

  • ARG63780 anti-Caveolin 1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human heart tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63780 anti-Caveolin 1 antibody at 5 µg/ml dilution followed by AP-staining.

  • ARG63780 anti-Caveolin 1 antibody WB image

    Western blot: 35 µg of A431 (A) and A549 (B) cell lysates (in RIPA buffer) stained with ARG63780 anti-Caveolin 1 antibody at 0.003 µg/ml (A) and 0.001 µg/ml (B) dilutions and incubated at RT for 1 hour.

  • ARG63780 anti-Caveolin 1 antibody FACS image

    Flow Cytometry: Paraformaldehyde-fixed HeLa cells permeabilized with 0.5% Triton. Cells were stained with ARG63780 anti-Caveolin 1 antibody (blue line) at 10 µg/ml dilution and overnight, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).

  • ARG63780 anti-Caveolin 1 antibody ICC/IF image

    Immunofluorescence: Paraformaldehyde fixed U2OS cells permeabilized with 0.15% Triton. Cells were stained with ARG63780 anti-Caveolin 1 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.

  • ARG63780 anti-Caveolin 1 antibody WB image

    Western blot: 35 µg of Human heart lysate (in RIPA buffer) stained with ARG63780 anti-Caveolin 1 antibody at 0.003 µg/ml dilution and incubated at RT for 1 hour.

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