anti-Caveolin 2 antibody
Key features and details
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- 反应物种:
- 应用:
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- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
CAT.NO. : ARG42497
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Product Details
概述
| 产品描述 | Goat Polyclonal antibody recognizes Caveolin 2 |
|---|---|
| 反应物种 | Hu, Ms, Rat, Dog, Mk |
| 应用 | ICC/IF, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Caveolin 2 |
| 抗原物种 | Human |
| 抗原 | Recombinant peptide within aa. 50 to the N-terminus of Human Caveolin 2. |
| 偶联标记 | Un-conjugated |
| 別名 | Caveolin-2; CAV |
应用说明
| 应用建议 |
| ||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | HaCaT | ||||||
| 实际分子量 | ~ 20 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.05% Sodium azide and 20% Glycerol. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 20% Glycerol |
| 浓度 | 3 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CAV2 |
| 全名 | caveolin 2 |
| 背景介绍 | The protein encoded by this gene is a major component of the inner surface of caveolae, small invaginations of the plasma membrane, and is involved in essential cellular functions, including signal transduction, lipid metabolism, cellular growth control and apoptosis. This protein may function as a tumor suppressor. This gene and related family member (CAV1) are located next to each other on chromosome 7, and express colocalizing proteins that form a stable hetero-oligomeric complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. Additional isoforms resulting from the use of alternate in-frame translation initiation codons have also been described, and shown to have preferential localization in the cell (PMID:11238462). [provided by RefSeq, May 2011] |
| 生物功能 | May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression (By similarity). [UniProt] |
| 细胞定位 | Nucleus. Cytoplasm. Golgi apparatus membrane. Cell membrane. Membrane, caveola. Note=Potential hairpin-like structure in the membrane. Membrane protein of caveolae. pTyr19 form is enriched at sites of cell-cell contact and is translocated to the nucleus in complex with MAPK1 in response to insulin. pTyr27 form is located both in the cytoplasm and plasma membrane. CAV1-mediated pSer23 form locates to the plasma membrane. pSer36 form resides in intracellular compartments. [UniProt] |
| 预测分子量 | 18 kDa |
| 翻译后修饰 | Phosphorylated on serine and tyrosine residues. CAV1 promotes phosphorylation on Ser-23 which then targets the complex to the plasma membrane, lipid rafts and caveolae. Phosphorylation on Ser-36 appears to modulate mitosis in endothelial cells (By similarity). Phosphorylation on both Tyr-19 and Tyr-27 is required for insulin-induced 'Ser-727' phosphorylation of STAT3 and its activation. Phosphorylation on Tyr-19 is required for insulin-induced phosphorylation of MAPK1 and DNA binding of STAT3. Tyrosine phosphorylation is induced by both EGF and insulin (By similarity). [UniProt] |
检测图片 (2)
ARG42497 anti-Caveolin 2 antibody ICC/IF image
Immunofluorescence: NIH/3T3 cells were fixed with methanol and permeabilized with 0.1% Saponin. Cells were stained with ARG42497 anti-Caveolin 2 antibody (green) at 1:100 dilution. Nuclear staining (blue).
ARG42497 anti-Caveolin 2 antibody WB image
Western blot: Caveolin 2-GFP or Caveolin 1-GFP transfected HEK293 cells and HaCaT cells. 50 µg of transfected HEK293 and 100 µg of HaCaT cell lysates stained with ARG42497 anti-Caveolin 2 antibody at 1:1000 dilution.
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