anti-CCL20 / MIP3 alpha antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes CCL20 / MIP3 alpha
  • 反应物种: Hu
  • 应用: ELISA, IHC-P, Neut, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: CCL20 / MIP3 alpha
  • 抗原物种: Human
  • 抗原: E.coli derived Recombinant Human MIP-3α (CCL20).(ASNFDCCLGY TDRILHPKFI VGFTRQLANE GCDINAIIFH TKKKLSVCAN PKQTWVKYIV RLLSKKVKNM)
  • Brand:
CAT.NO. : ARG56704
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Size:
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes CCL20 / MIP3 alpha
反应物种Hu
应用ELISA, IHC-P, Neut, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称CCL20 / MIP3 alpha
抗原物种Human
抗原E.coli derived Recombinant Human MIP-3α (CCL20).
(ASNFDCCLGY TDRILHPKFI VGFTRQLANE GCDINAIIFH TKKKLSVCAN PKQTWVKYIV RLLSKKVKNM)
偶联标记Un-conjugated
別名ST38; CC chemokine LARC; Exodus; Macrophage inflammatory protein 3 alpha; 2-70; MIP-3a; Beta-chemokine exodus-1; SCYA20; MIP-3-alpha; LARC; MIP3A; 1-64; Small-inducible cytokine A20; 1-67; CKb4; C-C motif chemokine 20; Liver and activation-regulated chemokine
应用说明
应用建议
应用推荐稀释比
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56813 as a detection antibody
IHC-P0.5 µg/ml
NeutAssay-dependent (lot-specific)
WB0.1 - 0.2 µg/ml
应用说明Neutralizing: The application for neutralizing is lot-specific, please contact info@arigobio.com for the detail.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Affinity purification with immunogen.
缓冲液PBS (pH 7.2)
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 6364 Human CCL20

Swiss-port # P78556 Human C-C motif chemokine 20

基因名称CCL20
全名chemokine (C-C motif) ligand 20
背景介绍This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene displays chemotactic activity for lymphocytes and can repress proliferation of myeloid progenitors. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]
生物功能Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213. [UniProt]
预测分子量11 kDa
翻译后修饰C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
检测图片 (4)
  • ARG56704 anti-CCL20 / MIP3 alpha antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human colon/rectum adenocarcinoma tissue. The recommended ARG56704 anti-CCL20 / MIP3 alpha antibody concentration is 0.5 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.

  • ARG56704 anti-CCL20 / MIP3 alpha antibody WB image

    Western blot: 250 - 0.24 ng of HumanMIP-3α stained with ARG56704 anti-CCL20 / MIP3 alpha antibody, under reducing conditions.

  • ARG56704 anti-CCL20 / MIP3 alpha antibody WB image

    Western blot: 250 - 0.24 ng of HumanMIP-3α stained with ARG56704 anti-CCL20 / MIP3 alpha antibody, under non-reducing conditions.

  • ARG56704 anti-CCL20 / MIP3 alpha antibody standard curve image

    Sandwich ELISA: ARG56704 anti-CCL20 / MIP3 alpha antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56813 anti-CCL20 antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.

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