anti-CD11b antibody [M1/70] (Functional Grade)
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Key features and details
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Product Details
| 产品描述 | Functional grade and low endotoxin Rat Monoclonal antibody [M1/70] recognizes CD11b |
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| 反应物种 | Hu, Ms, NHuPrm, Rb |
| 应用 | CyTOF®-candidate, FACS, FuncSt, IHC-Fr, IP |
| 特异性 | The clone M1/70 detects CD11b (integrin alphaM subunit), a type I transmembrane protein mainly expressed on monocytes/macrophages, granulocytes and NK-cells, which associates with CD18 to form Mac-1 integrin that plays important role in cell-cell interactions. |
| 宿主 | Rat |
| 克隆 | Monoclonal |
| 克隆号 | M1/70 |
| 同位型 | IgG2b |
| 靶点名称 | CD11b |
| 抗原物种 | Mouse |
| 抗原 | B10 mouse spleen cells enriched for T cells |
| 偶联标记 | Un-conjugated |
| 別名 | MAC1A; CR3A; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Integrin alpha-M; MAC-1; CD11 antigen-like family member B; Leukocyte adhesion receptor MO1; MO1A; SLEB6; Neutrophil adherence receptor; CD antigen CD11b; CD11B |
| 应用建议 |
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| 应用说明 | Functional studies: In vitro blocking of CD11b. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 纯化说明 | 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
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| 背景介绍 | CD11b (integrin alpha M chain): Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
| 生物功能 | CD11b: Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed:9558116, PubMed:20008295). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed:28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed:21193407). May regulate phagocytosis-induced apoptosis in extravasated neutrophils. May play a role in mast cell development. Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development. [UniProt] |
| 产品亮点 | Related products: CD11b antibodies; CD11b Duos / Panels; Anti-Rat Rat secondary antibodies; Related news: CyTOF-candidate Antibodies New antibody panels and duos for Tumor immune microenvironment Anti-SerpinB9 therapy, a new strategy for cancer therapy |
| 研究领域 | MDSC Marker antibody; Myeloid-derived suppressor cell antibody |
| 预测分子量 | 127 kDa |
ARG62718 anti-CD11b antibody [M1/70] (Functional Grade) FACS image
Flow Cytometry: Murine splenocyte suspension stained with ARG62718 anti-CD11b antibody [M1/70] (Functional Grade) at 0.6 µg/ml dilution, followed by APC-conjugated Donkey anti-Rat antibody.
ARG62718 anti-CD11b antibody [M1/70] (Functional Grade) FACS image
Flow Cytometry: Separation of murine CD11b positive myeloid cells (red-filled) from murine CD11b negative lymphocytes (black-dashed). Murine splenocyte suspension stained with ARG62718 anti-CD11b antibody [M1/70] (Functional Grade) at 0.6 µg/ml dilution, followed by APC-conjugated Donkey anti-Rat antibody.
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