anti-CD137 antibody [4-1BB/3201]

Key features and details

产品描述: Mouse Monoclonal antibody [4-1BB/3201] recognizes CD137
反应物种: Hu
应用: FACS, ICC/IF, IHC-P, WB
宿主: Mouse
克隆: Monoclonal
克隆号: 4-1BB/3201
同位型: IgG1, kappa
靶点名称: CD137
抗原物种: Human

Brand:

CAT.NO. : ARG43309

US＄ Please choose

Size:

50 μg

Trail, Bulk size or Custom requests Please contact us

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

概述

产品描述	Mouse Monoclonal antibody [4-1BB/3201] recognizes CD137
反应物种	Hu
应用	FACS, ICC/IF, IHC-P, WB
宿主	Mouse
克隆	Monoclonal
克隆号	4-1BB/3201
同位型	IgG1, kappa
靶点名称	CD137
抗原物种	Human
抗原	Recombinant protein fragment within aa. 19-188 of Human CD137.
偶联标记	Un-conjugated
別名	ILA; CD137; T-cell antigen 4-1BB homolog; CDw137; T-cell antigen ILA; 4-1BB; CD antigen CD137; Tumor necrosis factor receptor superfamily member 9; 4-1BB ligand receptor

应用说明

应用建议

应用	推荐稀释比
FACS	1 - 2 µg/10^6 cells
ICC/IF	1 - 5 µg/ml
IHC-P	1 - 5 µg/ml
WB	1-2ug/ml

应用说明

IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Tris with 1 mM EDTA (pH 9.0) for 10-20 min, followed by cooling at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein G.
缓冲液	PBS, 0.05% Sodium azide and 0.1 mg/ml BSA.
抗菌剂	0.05% Sodium azide
稳定剂	0.1 mg/ml BSA
浓度	0.2 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 3604 Human TNFRSF9 Swiss-port # Q07011 Human Tumor necrosis factor receptor superfamily member 9
基因名称	TNFRSF9
全名	tumor necrosis factor receptor superfamily, member 9
背景介绍	The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contributes to the clonal expansion, survival, and development of T cells. It can also induce proliferation in peripheral monocytes, enhance T cell apoptosis induced by TCR/CD3 triggered activation, and regulate CD28 co-stimulation to promote Th1 cell responses. The expression of this receptor is induced by lymphocyte activation. TRAF adaptor proteins have been shown to bind to this receptor and transduce the signals leading to activation of NF-kappaB. [provided by RefSeq, Jul 2008]
生物功能	Receptor for TNFSF9/4-1BBL. Possibly active during T cell activation. [UniProt]
细胞定位	Membrane; Single-pass type I membrane protein. [UniProt]
预测分子量	28 kDa

检测图片 (7)

ARG43309 anti-CD137 antibody [4-1BB/3201] ICC/IF image
Immunofluorescence: MeOH-fixed HEK293 cells stained with ARG43309 anti-CD137 antibody [4-1BB/3201] (green) and Reddot nuclear stain (red).
ARG43309 anti-CD137 antibody [4-1BB/3201] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human spleen tissue. Antigen Retrieval: Boil tissue section in 10 mM Tris with 1 mM EDTA (pH 9.0) for 10-20 min, followed by cooling at RT. The tissue section was stained with ARG43309 anti-CD137 antibody [4-1BB/3201].
ARG43309 anti-CD137 antibody [4-1BB/3201] WB image
Western blot: Human Kidney stained with ARG43309 anti-CD137 antibody [4-1BB/3201] .
ARG43309 anti-CD137 antibody [4-1BB/3201] FACS image
Flow Cytometry: MeOH-fixed HEK293 cells stained with ARG43309 anti-CD137 antibody [4-1BB/3201] (blue) or isotype control (red).
ARG43309 anti-CD137 antibody [4-1BB/3201] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human cerebellum tissue. Antigen Retrieval: Boil tissue section in 10 mM Tris with 1 mM EDTA (pH 9.0) for 10-20 min, followed by cooling at RT. The tissue section was stained with ARG43309 anti-CD137 antibody [4-1BB/3201].
ARG43309 anti-CD137 antibody [4-1BB/3201] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human renal cell carcinoma. Antigen Retrieval: Boil tissue section in 10 mM Tris with 1 mM EDTA (pH 9.0) for 10-20 min, followed by cooling at RT. The tissue section was stained with ARG43309 anti-CD137 antibody [4-1BB/3201].
ARG43309 anti-CD137 antibody [4-1BB/3201] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human renal cell carcinoma. Antigen Retrieval: Boil tissue section in 10 mM Tris with 1 mM EDTA (pH 9.0) for 10-20 min, followed by cooling at RT. The tissue section was stained with ARG43309 anti-CD137 antibody [4-1BB/3201].