anti-CD14 antibody [MEM-15] (APC)
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CAT.NO. : ARG53766
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Product Details
概述
| 产品描述 | APC-conjugated Mouse Monoclonal antibody [MEM-15] recognizes CD14 |
|---|---|
| 反应物种 | Hu, NHuPrm |
| 应用 | FACS |
| 特异性 | The clone MEM-15 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages. MEM-15 also reacts with soluble forms of CD14 found in serum and in the urine of some nephrotic patients. HLDA III; WS Code M 252 HLDA IV; WS Code M 113 HLDA IV; WS Code NL 90 HLDA IV; WS Code T 53 HLDA V; WS Code M MA086 HLDA VI; WS Code M MA94 |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | MEM-15 |
| 同位型 | IgG1 |
| 靶点名称 | CD14 |
| 抗原物种 | Human |
| 抗原 | A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria. |
| 偶联标记 | APC |
| 別名 | CD antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; Monocyte differentiation antigen CD14 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化说明 | The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| 缓冲液 | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| 抗菌剂 | 15 mM Sodium azide |
| 稳定剂 | 0.2% (w/v) high-grade protease free BSA |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P08571 Human Monocyte differentiation antigen CD14 |
|---|---|
| 基因名称 | CD14 |
| 全名 | CD14 molecule |
| 背景介绍 | CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor; it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes. |
| 生物功能 | In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30313 General Lymphocyte Marker Antibody Panel (CD3, CD14, CD16, CD19, CD56) Related products: CD14 antibodies; CD14 ELISA Kits; CD14 Duos / Panels; Anti-Mouse IgG secondary antibodies; |
| 研究领域 | Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Macrophages and neutrophils antibody |
| 预测分子量 | 40 kDa |
| 翻译后修饰 | N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. |
检测图片 (1)
ARG53766 anti-CD14 antibody [MEM-15] (APC) FACS image
Flow Cytometry: Human peripheral blood cells stained with ARG53766 anti-CD14 antibody [MEM-15] (APC).
参考文献
克隆号文献
Targeting the giant cell tumor stromal cell: functional characterization and a novel therapeutic strategy.
FACS / Human
Yersinia V-antigen exploits toll-like receptor 2 and CD14 for interleukin 10-mediated immunosuppression.
CD14 is expressed and released as soluble CD14 by human intestinal epithelial cells in vitro: lipopolysaccharide activation of epithelial cells revisited.
Soluble CD14 from urine copurifies with a potent inducer of cytokines.
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