anti-CD147 antibody [MEM-M6/6] (low endotoxin)
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- 靶点名称:
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CAT.NO. : ARG65506
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Product Details
概述
| 产品描述 | Azide free and low endotoxin Mouse Monoclonal antibody [MEM-M6/6] recognizes CD147 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, FuncSt, WB |
| 特异性 | The clone MEM-M6/6 recognizes Ig domain D2 (membrane proximal) of CD147 (Neurothelin), a 50-60 kDa type I transmembrane glycoprotein primarily expressed on all leukocytes, red blood cells, platelets and endothelial cells; it is not expressed by resting lymphocytes. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | MEM-M6/6 |
| 同位型 | IgG1 |
| 靶点名称 | CD147 |
| 抗原物种 | Human |
| 抗原 | Protein A-CR purified soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the hinge region, CH2-and CH3 domain of human IgG1 (CD147Rg is secreted by transfectants as a dimmer). |
| 偶联标记 | Un-conjugated |
| 別名 | OK; Tumor cell-derived collagenase stimulatory factor; Leukocyte activation antigen M6; TCSF; Extracellular matrix metalloproteinase inducer; OK blood group antigen; CD147; Basigin; M6; EMMPRIN; Collagenase stimulatory factor; CD antigen CD147; 5F7 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-reducing condition. Functional studies: The clone MEM-M6/6 (high-affinity mAb of unique epitope specificity) inhibits anti-CD3-induced T cell activation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | WB: 293 cells |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 纯化说明 | 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | BSG |
| 全名 | basigin (Ok blood group) |
| 背景介绍 | CD147 (basigin, neurothelin, OX-47, 5A11, CE9, M6) also known as EMMPRIN (extracellular matrix metalloproteinase inducer) or TCSF (tumour cell-derived collagenase-stimulatory factor) is an ubiquitously expressed cell surface protein with multiple glycosylated forms. The highest level of CD147 expression is on metabolically active cells, such as lymphoblasts, inflammatory cells, brown adipocytes and malignant tumour cells. CD147 has multiple functions, including facilitating of cell surface expression of monocarboxylate transporter proteins and extracellular matrix metalloproteinases, regulation of integrin functions, it plays roles in cell development and activation, fetal development or retinal function. |
| 生物功能 | Plays an important role in targeting the monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to the plasma membrane. Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. [UniProt] |
| 研究领域 | Cancer antibody; Immune System antibody; Metabolism antibody; Neuroscience antibody |
| 预测分子量 | 42 kDa |
| 翻译后修饰 | N-glycosylated. |
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