anti-CD152 / CTLA4 antibody [4C8]
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Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
Product Details
| 产品描述 | Mouse Monoclonal antibody [4C8] recognizes CD152 / CTLA4 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 4C8 |
| 同位型 | IgG1, kappa |
| 靶点名称 | CD152 / CTLA4 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 36-161 of Human CD152 / CTLA4 |
| 偶联标记 | Un-conjugated |
| 別名 | GRD4; CTLA-4; CELIAC3; CD; Cytotoxic T-lymphocyte-associated antigen 4; CD152; GSE; CD antigen CD152; Cytotoxic T-lymphocyte protein 4; ALPS5; IDDM12 |
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
|---|---|
| 基因名称 | CTLA4 |
| 全名 | cytotoxic T-lymphocyte-associated protein 4 |
| 背景介绍 | This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. [provided by RefSeq, Jul 2008] |
| 生物功能 | Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. [UniProt] |
| 产品亮点 | Related products: CD152 antibodies; Anti-Mouse IgG secondary antibodies; Related news: New PD-1 ELISA Kit, excellent for preclinical studies or pharmatheutical development Why anti-PD-1/PD-L1 therapy doesn’t work? |
| 预测分子量 | 25 kDa |
| 翻译后修饰 | N-glycosylation is important for dimerization. Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface. |
ARG57120 anti-CD152 / CTLA4 antibody [4C8] ICC/IF image
Immunofluorescence: Jurkat cells stained with ARG57120 anti-CD152 / CTLA4 antibody [4C8] (green) at 1:100 dilution. DAPI (blue) for nucleus staining.
ARG57120 anti-CD152 / CTLA4 antibody [4C8] FACS image
Flow Cytometry: HeLa cells stained with ARG57120 anti-CD152 / CTLA4 antibody [4C8] at 2-5 µg / 10^6 cells (red). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
ARG57120 anti-CD152 / CTLA4 antibody [4C8] WB image
Western blot: 40 µg of Mouse liver, TF-1, HepG2 and WiDr cell lysates stained with ARG57120 anti-CD152 / CTLA4 antibody [4C8] at 1:1000 dilution.
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