anti-CD152 / CTLA4 antibody [BNI3]
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Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
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- 同位型:
- 靶点名称:
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Product Details
| 产品描述 | Mouse Monoclonal antibody [BNI3] recognizes CD152 / CTLA4 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, IHC-Fr, IP |
| 特异性 | The clone BNI3 recognizes human CD152 / CTLA4, an approximately 45 kDa type I transmembrane protein serving as a negative regulator of T cell responses. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | BNI3 |
| 同位型 | IgG2a |
| 靶点名称 | CD152 / CTLA4 |
| 抗原物种 | Human |
| 抗原 | Human CD152-IgG heavy chain fusion protein |
| 偶联标记 | Un-conjugated |
| 別名 | GRD4; CTLA-4; CELIAC3; CD; Cytotoxic T-lymphocyte-associated antigen 4; CD152; GSE; CD antigen CD152; Cytotoxic T-lymphocyte protein 4; ALPS5; IDDM12 |
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | FACS: Intracellular staining. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from cell culture supernatant by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
|---|---|
| 基因名称 | CTLA4 |
| 全名 | cytotoxic T-lymphocyte-associated protein 4 |
| 背景介绍 | CD152 / CTLA-4 is a homodimeric transmembrane protein similar to CD28 and binding the same ligands, i.e. CD80 (B7.1) and CD86 (B7.2), but with higher affinity. Unlike CD28 with important costimulating functions, CD152 acts as an important inhibitory receptor essential for modulation of the immune system. CD152 / CTLA-4 becomes transiently expressed on activated T cells and its malfunction can cause autoimmune diseases, such as insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, or thyroid-associated orbitopathy. |
| 生物功能 | Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. [UniProt] |
| 研究领域 | Developmental Biology antibody; Immune System antibody |
| 预测分子量 | 25 kDa |
| 翻译后修饰 | N-glycosylation is important for dimerization. Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface. |
ARG65419 anti-CD152 / CTLA4 antibody [BNI3] FACS image
Flow Cytometry: PHA stimulated human peripheral whole blood stained with ARG65419 anti-CD152 / CTLA4 antibody [BNI3] at 10 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
ARG65419 anti-CD152 / CTLA4 antibody [BNI3] FACS image
Flow Cytometry: PHA stimulated human lymphocytes stained with ARG65419 anti-CD152 / CTLA4 antibody [BNI3] at 10 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody. Cells were co-stained with ARG53820 anti-CD3 antibody [UCHT1] (PE) (20 µl reagent / 100 µl of peripheral whole blood).
ARG65419 anti-CD152 / CTLA4 antibody [BNI3] FACS image
Flow Cytometry: Separation of human CD152 positive CD3 positive lymphocytes (red-filled) from CD152 negative CD3 negative lymphocytes (black-dashed). Human peripheral whole blood stained with ARG65419 anti-CD152 / CTLA4 antibody [BNI3] at 10 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
克隆号文献
Role of CTLA4 in the proliferation and survival of chronic lymphocytic leukemia.
WB / Human
Expanding the psoriasis disease profile: interrogation of the skin and serum of patients with moderate-to-severe psoriasis.
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A screen for endocytic motifs.
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