anti-CD154 / CD40L antibody [CD40LG/2761]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [CD40LG/2761] recognizes CD154 / CD40L
  • 反应物种: Hu
  • 应用: FACS, ICC/IF, IHC-P
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: CD40LG/2761
  • 同位型: IgG2b, kappa
  • 靶点名称: CD154 / CD40L
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG41609
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Product Details
概述
产品描述Mouse Monoclonal antibody [CD40LG/2761] recognizes CD154 / CD40L
反应物种Hu
应用FACS, ICC/IF, IHC-P
宿主Mouse
克隆Monoclonal
克隆号CD40LG/2761
同位型IgG2b, kappa
靶点名称CD154 / CD40L
抗原物种Human
抗原Protein fragment corresponding to aa. 108-261 of Human CD154.
偶联标记Un-conjugated
別名TNFSF5; IMD3; T-cell antigen Gp39; HIGM1; CD40-L; gp39; CD40 ligand; Tumor necrosis factor ligand superfamily member 5; CD40L; CD154; TRAP; CD antigen CD154; hCD40L; IGM; T-BAM; TNF-related activation protein
应用说明
应用建议
应用推荐稀释比
FACS1 - 2 µg/10^6 cells
ICC/IF1 - 2 µg/ml
IHC-P1 - 2 µg/ml
应用说明IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purification with Protein G.
缓冲液PBS, 0.05% Sodium azide and 0.1 mg/ml BSA.
抗菌剂0.05% Sodium azide
稳定剂0.1 mg/ml BSA
浓度0.2 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 959 Human CD40LG

Swiss-port # P29965 Human CD40 ligand

基因名称CD40LG
全名CD40 ligand
背景介绍The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]
生物功能Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.

Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. [UniProt]
细胞定位Cell membrane; Single-pass type II membrane protein. Cell surface. CD40 ligand, soluble form: Secreted. [UniProt]
预测分子量29 kDa
翻译后修饰The soluble form derives from the membrane form by proteolytic processing.

N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.

Not O-glycosylated. [UniProt]
检测图片 (5)
  • ARG41609 anti-CD154 / CD40L antibody [CD40LG/2761] ICC/IF image

    Immunofluorescence: Paraformaldehyde-fixed Jurkat cells stained with ARG41609 anti-CD154 / CD40L antibody [CD40LG/2761] (green) and Reddot nuclear stain (red).

  • ARG41609 anti-CD154 / CD40L antibody [CD40LG/2761] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human spleen tissue stained with ARG41609 anti-CD154 / CD40L antibody [CD40LG/2761]. Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min followed by cooling at RT for 20 min.

  • ARG41609 anti-CD154 / CD40L antibody [CD40LG/2761] FACS image

    Flow Cytometry: Jurkat cells stained with ARG41609 anti-CD154 / CD40L antibody [CD40LG/2761] (blue) or isotype control antibody (red), followed by incubation with FITC labelled secondary antibody.

  • ARG41609 anti-CD154 / CD40L antibody [CD40LG/2761] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human spleen tissue stained with ARG41609 anti-CD154 / CD40L antibody [CD40LG/2761]. Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min followed by cooling at RT for 20 min.

  • ARG41609 anti-CD154 / CD40L antibody [CD40LG/2761] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human tonsil tissue stained with ARG41609 anti-CD154 / CD40L antibody [CD40LG/2761]. Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min followed by cooling at RT for 20 min.

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