anti-CD16 antibody [LNK16] (FITC)

Key features and details

  • 产品描述: FITC-conjugated Mouse Monoclonal antibody [LNK16] recognizes CD16
  • 反应物种: Hu, NHuPrm
  • 应用: FACS
  • 特异性: The clone LNK16 reacts with CD16, a low affinity receptor for aggregated IgG (FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA; 50-65 kDa; expressed on NK-cells, monocytes and macrophages) and CD16b (FcgammaRIIIB; 48 kDa; mainly expressed on neutrophils).HLDA V; WS Code M MA069HLDA V; WS Code NK NK50
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: LNK16
  • 同位型: IgG1
  • 靶点名称: CD16
  • Brand:
CAT.NO. : ARG62744
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Product Details
概述
产品描述FITC-conjugated Mouse Monoclonal antibody [LNK16] recognizes CD16
反应物种Hu, NHuPrm
应用FACS
特异性The clone LNK16 reacts with CD16, a low affinity receptor for aggregated IgG (FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA; 50-65 kDa; expressed on NK-cells, monocytes and macrophages) and CD16b (FcgammaRIIIB; 48 kDa; mainly expressed on neutrophils).
HLDA V; WS Code M MA069
HLDA V; WS Code NK NK50
宿主Mouse
克隆Monoclonal
克隆号LNK16
同位型IgG1
靶点名称CD16
抗原物种Human
抗原Normal human peripheral blood granulocytes
偶联标记FITC
別名FCRIIIA; FcRIIIa; CD antigen CD16a; Fc-gamma RIII-alpha; FCR-10; FcR-10; FCRIII; FCG3; Low affinity immunoglobulin gamma Fc region receptor III-A; FCGRIII; CD16; Fc-gamma RIIIa; IgG Fc receptor III-2; IMD20; CD16A; IGFR3; CD16a antigen; FCGR3; FcRIII; Fc-gamma RIII
应用说明
应用建议
应用推荐稀释比
FACS20 µl / 10^6 cells
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化说明The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.
缓冲液PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
抗菌剂15 mM Sodium azide
稳定剂0.2% (w/v) high-grade protease free BSA
存放说明Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 2214 Human FCGR3A

Swiss-port # P08637 Human Low affinity immunoglobulin gamma Fc region receptor III-A

基因名称FCGR3A
全名Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
背景介绍CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms – FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.
生物功能Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. [UniProt]
产品亮点Related products:
CD16 antibodies; CD16 ELISA Kits; CD16 Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
Tumor-Infiltrating Lymphocytes (TILs)
研究领域Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Natural killer cells antibody
预测分子量29 kDa
翻译后修饰Glycosylated. Contains high mannose- and complex-type oligosaccharides. Glycosylation at Asn-180 is mandatory for high affinity binding to the Fc and for discrimination between fucosylated and afucosylated IgG glycoforms.
The soluble form is produced by a proteolytic cleavage.
检测图片 (3)
  • ARG62744 anti-CD16 antibody [LNK16] (FITC) FACS image

    Flow Cytometry: Human peripheral whole blood stained with ARG62744 anti-CD16 antibody [LNK16] (FITC) (20 µl reagent / 100 µl of peripheral whole blood).

  • ARG62744 anti-CD16 antibody [LNK16] (FITC) FACS image

    Flow Cytometry: Human Lymphocytes stained with ARG62744 anti-CD16 antibody [LNK16] (FITC) (20 µl reagent / 100 µl of peripheral whole blood) and ARG54302 anti-CD3 epsilon antibody [UCHT1] (APC) (10 µl reagent / 100 µl of peripheral whole blood).

  • ARG62744 anti-CD16 antibody [LNK16] (FITC) FACS image

    Flow Cytometry: Separation of Human CD3 negative CD16 positive Lymphocytes (red) from Human CD3 positive CD16 negative Lymphocytes (black-dashed). Human peripheral whole blood stained with ARG62744 anti-CD16 antibody [LNK16] (FITC) (20 µl reagent / 100 µl of peripheral whole blood).

参考文献

克隆号文献

Human macrophages kill human mesangial cells by Fas-L-induced apoptosis when triggered by antibody via CD16.

Boyle JJ et al.
Clin Exp Immunol.,  (2004)

publication_link

 

hr_line

The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding.

Tamm A et al.
J Immunol.,  (1996)

publication_link

 

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