anti-CD16 antibody [MEM-154] (low endotoxin)
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Key features and details
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Product Details
| 产品描述 | Azide free and low endotoxin Mouse Monoclonal antibody [MEM-154] recognizes CD16 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, FuncSt, IP, WB |
| 特异性 | The antibody MEM-154 reacts with an extracellular epitope on CD16 antigen that is residing in proximity to FG loop (probably BC or C'E loop). CD16 is a low affinity receptor for aggregated IgG (FcgammaRIII antigen). The antibody MEM-154 reacts with CD16+ granulocytes. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | MEM-154 |
| 同位型 | IgG1 |
| 靶点名称 | CD16 |
| 抗原物种 | Human |
| 抗原 | Human granulocytes. |
| 偶联标记 | Un-conjugated |
| 別名 | FCRIIIA; FcRIIIa; CD antigen CD16a; Fc-gamma RIII-alpha; FCR-10; FcR-10; FCRIII; FCG3; Low affinity immunoglobulin gamma Fc region receptor III-A; FCGRIII; CD16; Fc-gamma RIIIa; IgG Fc receptor III-2; IMD20; CD16A; IGFR3; CD16a antigen; FCGR3; FcRIII; Fc-gamma RIII |
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 纯化说明 | 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein. |
| 缓冲液 | PBS |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | Swiss-port # P08637 Human Low affinity immunoglobulin gamma Fc region receptor III-A |
|---|---|
| 基因名称 | FCGR3A |
| 全名 | Fc fragment of IgG, low affinity IIIa, receptor (CD16a) |
| 背景介绍 | This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type I membrane protein. Secreted. Note=Exists also as a soluble receptor. [UniProt] |
| 预测分子量 | 29 kDa |
| 翻译后修饰 | Glycosylated. Contains high mannose- and complex-type oligosaccharides. Glycosylation at Asn-180 is mandatory for high affinity binding to the Fc and for discrimination between fucosylated and afucosylated IgG glycoforms. The soluble form is produced by a proteolytic cleavage. [UniProt] |
ARG65489 anti-CD16 antibody [MEM-154] (low endotoxin) FACS image
Flow Cytometry: Human peripheral blood stained with ARG65489 anti-CD16 antibody [MEM-154] (low endotoxin) at 2 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
ARG65489 anti-CD16 antibody [MEM-154] (low endotoxin) FACS image
Flow Cytometry: Separation of Human neutrophil granulocytes (red-filled) from CD16 negative lymphocytes (black-dashed). Human peripheral whole blood stained with ARG65489 anti-CD16 antibody [MEM-154] (low endotoxin) at 2 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
克隆号文献
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