anti-CD161 antibody [HP-3G10] (PE)

产品描述: PE-conjugated Mouse Monoclonal antibody [HP-3G10] recognizes CD161
反应物种: Hu, NHuPrm
应用: FACS
特异性: The clone HP-3G10 recognizes CD161, a type II transmembrane C-type lectin receptor, expressed on the plasma membrane of NK cells, dendritic cells, activated monocytes and a subset of T cells as a disulphide-linked homodimer.
宿主: Mouse
克隆: Monoclonal
克隆号: HP-3G10
同位型: IgG1
靶点名称: CD161

CAT.NO. : ARG54259

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Size:

50 tests

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

概述

产品描述	PE-conjugated Mouse Monoclonal antibody [HP-3G10] recognizes CD161
反应物种	Hu, NHuPrm
应用	FACS
特异性	The clone HP-3G10 recognizes CD161, a type II transmembrane C-type lectin receptor, expressed on the plasma membrane of NK cells, dendritic cells, activated monocytes and a subset of T cells as a disulphide-linked homodimer.
宿主	Mouse
克隆	Monoclonal
克隆号	HP-3G10
同位型	IgG1
靶点名称	CD161
抗原物种	Human
抗原	human NK cells
偶联标记	PE
別名	CLEC5B; CD antigen CD161; CD161; NKR-P1; NKR-P1A; Killer cell lectin-like receptor subfamily B member 1; NKRP1A; NKR; HNKR-P1a; Natural killer cell surface protein P1A; C-type lectin domain family 5 member B; hNKR-P1A

应用说明

应用建议

应用	推荐稀释比
FACS	10 µl / 10^6 cells

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化说明	The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
缓冲液	PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
抗菌剂	15 mM Sodium azide
稳定剂	0.2% (w/v) high-grade protease free BSA
存放说明	Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 3820 Human KLRB1 Swiss-port # Q12918 Human Killer cell lectin-like receptor subfamily B member 1
基因名称	KLRB1
全名	killer cell lectin-like receptor subfamily B, member 1
背景介绍	CD161, also known as Nkrp1 (natural killer receptor protein 1) or Klrb1 (killer cell lectin-like receptor subfamily b member 1), is a disulphide-linked homodimeric receptor, which is involved in regulation of NK cell and NKT cell function. It is expressed on a majority of NK cells, NKT cells, and e.g. Th17 cells and CD3+ thymocytes.Although rat CD161 has three isoforms (a, b, c), the human CD161 is expressed as one isoform.
生物功能	Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells. [UniProt]
研究领域	Developmental Biology antibody; Immune System antibody
预测分子量	25 kDa
翻译后修饰	N-glycosylated. Contains sialic acid residues.

检测图片 (1)

ARG54259 anti-CD161 antibody [HP-3G10] (PE) FACS image
Flow Cytometry: Human peripheral blood cells stained with ARG54259 anti-CD161 antibody [HP-3G10] (PE).

参考文献

克隆号文献

CD161 (human NKR-P1A) signaling in NK cells involves the activation of acid sphingomyelinase.

Pozo D et al.
J Immunol., (2006)