anti-CD18 / LFA1 beta antibody [IVA35]
![anti-CD18 / LFA1 beta antibody [IVA35]](/upload/image/products/ARG62754_FACS_2_201117_210_205.jpg)
![anti-CD18 / LFA1 beta antibody [IVA35]](/upload/image/products/ARG62754_IF_1.jpg)
![anti-CD18 / LFA1 beta antibody [IVA35]](/upload/image/products/ARG62754_FACS_1_201117.jpg)
![anti-CD18 / LFA1 beta antibody [IVA35]](/upload/image/products/ARG62754_FACS_2_201117.jpg)
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
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Product Details
| 产品描述 | Mouse Monoclonal antibody [IVA35] recognizes CD18 / LFA1 beta |
|---|---|
| 反应物种 | Bov |
| 应用 | FACS, ICC/IF, IP |
| 特异性 | The clone IVA35 reacts with CD18 antigen (bovine), a 90-95 kDa type I transmembrane protein (integrin) expressed on all leukocytes. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | IVA35 |
| 同位型 | IgG1 |
| 靶点名称 | CD18 / LFA1 beta |
| 抗原 | Bovine leukocytes. |
| 偶联标记 | Un-conjugated |
| 別名 | MF17; LAD; CD antigen CD18; MFI7; MAC-1; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; LCAMB; Integrin beta-2; Complement receptor C3 subunit beta; LFA-1; CD18 |
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from ascites by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
|---|---|
| 基因名称 | ITGB2 |
| 全名 | integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) |
| 背景介绍 | This gene encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
| 生物功能 | Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation (By similarity). Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. [UniProt] |
| 研究领域 | Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody |
| 预测分子量 | 85 kDa |
| 翻译后修饰 | Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters (PubMed:11700305). Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins (PubMed:11700305, PubMed:16301335). |
ARG62754 anti-CD18 / LFA1 beta antibody [IVA35] ICC/IF image
Immunofluorescence: Acetone / methanol-fixed Bovine peripheral blood (lymphocyte-enriched suspension) stained with ARG62754 anti-CD18 / LFA1 beta antibody [IVA35] (green), DAPI (blue) for nuclear staining.
ARG62754 anti-CD18 / LFA1 beta antibody [IVA35] FACS image
Flow Cytometry: Separation of stained bovine lymphocytes (red-filled) from unstained bovine lymphocytes (black-dashed). Cells were stained with ARG62754 anti-CD18 / LFA1 beta antibody [IVA35] at 10 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
ARG62754 anti-CD18 / LFA1 beta antibody [IVA35] FACS image
Flow Cytometry: Bovine peripheral whole blood stained with ARG62754 anti-CD18 / LFA1 beta antibody [IVA35] at 10 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
克隆号文献
Monoclonal antibodies specific for bovine CD18.
Nomenclature and characterization of leukocyte differentiation antigens in ruminants.
Proceedings of the 3rd workshop on ruminant leukocyte antigens. Davis, California, 16 July 1995.
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