anti-CD18 / LFA1 beta antibody [IVA35]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [IVA35] recognizes CD18 / LFA1 beta
  • 反应物种: Bov
  • 应用: FACS, ICC/IF, IP
  • 特异性: The clone IVA35 reacts with CD18 antigen (bovine), a 90-95 kDa type I transmembrane protein (integrin) expressed on all leukocytes.
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: IVA35
  • 同位型: IgG1
  • 靶点名称: CD18 / LFA1 beta
  • Brand:
CAT.NO. : ARG62754
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Product Details
概述
产品描述Mouse Monoclonal antibody [IVA35] recognizes CD18 / LFA1 beta
反应物种Bov
应用FACS, ICC/IF, IP
特异性The clone IVA35 reacts with CD18 antigen (bovine), a 90-95 kDa type I transmembrane protein (integrin) expressed on all leukocytes.
宿主Mouse
克隆Monoclonal
克隆号IVA35
同位型IgG1
靶点名称CD18 / LFA1 beta
抗原Bovine leukocytes.
偶联标记Un-conjugated
別名MF17; LAD; CD antigen CD18; MFI7; MAC-1; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; LCAMB; Integrin beta-2; Complement receptor C3 subunit beta; LFA-1; CD18
应用说明
应用建议
应用推荐稀释比
FACS1 - 4 µg/ml
ICC/IFAssay-dependent
IPAssay-dependent
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified from ascites by protein-A affinity chromatography.
纯度> 95% (by SDS-PAGE)
缓冲液PBS (pH 7.4) and 15 mM Sodium azide
抗菌剂15 mM Sodium azide
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 281877 Bovine ITGB2

Swiss-port # P32592 Bovine Integrin beta-2

基因名称ITGB2
全名integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
背景介绍This gene encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
生物功能Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation (By similarity). Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. [UniProt]
研究领域Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
预测分子量85 kDa
翻译后修饰Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters (PubMed:11700305). Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins (PubMed:11700305, PubMed:16301335).
检测图片 (3)
  • ARG62754 anti-CD18 / LFA1 beta antibody [IVA35] ICC/IF image

    Immunofluorescence: Acetone / methanol-fixed Bovine peripheral blood (lymphocyte-enriched suspension) stained with ARG62754 anti-CD18 / LFA1 beta antibody [IVA35] (green), DAPI (blue) for nuclear staining.

  • ARG62754 anti-CD18 / LFA1 beta antibody [IVA35] FACS image

    Flow Cytometry: Separation of stained bovine lymphocytes (red-filled) from unstained bovine lymphocytes (black-dashed). Cells were stained with ARG62754 anti-CD18 / LFA1 beta antibody [IVA35] at 10 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.

  • ARG62754 anti-CD18 / LFA1 beta antibody [IVA35] FACS image

    Flow Cytometry: Bovine peripheral whole blood stained with ARG62754 anti-CD18 / LFA1 beta antibody [IVA35] at 10 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.

参考文献

克隆号文献

Monoclonal antibodies specific for bovine CD18.

Dusinský R et al.
Folia Biol (Praha).,  (2001)

publication_link

 

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Nomenclature and characterization of leukocyte differentiation antigens in ruminants.

Naessens J et al.
Immunol Today.,  (1997)

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Proceedings of the 3rd workshop on ruminant leukocyte antigens. Davis, California, 16 July 1995.

[No authors listed] et al.
Vet Immunol Immunopathol.,  (1996)

publication_link

 

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