anti-CD18 / LFA1 beta antibody [M18 / 2] (FITC)

Key features and details

产品描述: FITC-conjugated Rat Monoclonal antibody [M18/2] recognizes CD18 / LFA1 beta
反应物种: Ms
不反应物种: Dog
应用: FACS
特异性: The clone M18/2 recognizes CD18 antigen (integrin beta2 subunit; beta2 integrin), a 95 kDa type I transmembrane protein expressed on all leukocytes.
宿主: Rat
克隆: Monoclonal
克隆号: M18/2
同位型: IgG2a

Brand:

CAT.NO. : ARG62755

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Size:

100 μg

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Product Details

概述

产品描述	FITC-conjugated Rat Monoclonal antibody [M18/2] recognizes CD18 / LFA1 beta
反应物种	Ms
不反应物种	Dog
应用	FACS
特异性	The clone M18/2 recognizes CD18 antigen (integrin beta2 subunit; beta2 integrin), a 95 kDa type I transmembrane protein expressed on all leukocytes.
宿主	Rat
克隆	Monoclonal
克隆号	M18/2
同位型	IgG2a
靶点名称	CD18 / LFA1 beta
抗原物种	Mouse
抗原	Murine cytotoxic T cell glycoproteins
偶联标记	FITC
別名	MF17; LAD; CD antigen CD18; MFI7; MAC-1; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; LCAMB; Integrin beta-2; Complement receptor C3 subunit beta; LFA-1; CD18

应用说明

应用建议

应用	推荐稀释比
FACS	1 - 2 µg/ml

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化说明	The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
缓冲液	PBS (pH 7.4) and 15 mM Sodium azide
抗菌剂	15 mM Sodium azide
浓度	0.5 mg/ml
存放说明	Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 16414 Mouse ITGB2 Swiss-port # P11835 Mouse Integrin beta-2
基因名称	Itgb2
全名	integrin beta 2
背景介绍	CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts.
生物功能	Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation. [UniProt]
研究领域	Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
预测分子量	85 kDa
翻译后修饰	Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters (PubMed:11700305). Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins (PubMed:11700305, PubMed:16301335).

检测图片 (1)

ARG62755 anti-CD18 / LFA1 beta antibody [M18 / 2] (FITC) FACS image
Flow Cytometry: Murine splenocytes stained with ARG62755 anti-CD18 / LFA1 beta antibody [M18 / 2] (FITC) at 3 µg/ml dilution (red). Blank sample (grey).

参考文献

克隆号文献

Neutrophil-selective CD18 silencing using RNA interference in vivo.

Cullere X et al.
Blood., (2008)