anti-CD203a antibody [PM18-7]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [PM18-7] recognizes CD203aThis antibody recognizes Porcine CD203a, originally clustered as SWC9 at the Second International Swine CD Workshop (Dominguez et al. 1998) and later identified as the Porcine homologue of Human ecto-nucleotidepyrophosphatase / phosphodiesterase 1 or ENPP1 (Petersen et al. 2007).Mouse anti Pig CD203a was originally reported to immunoprecipitate two bands, one of ~;205 kDa and one of ~130 kDa (Dominguez et al. 1998) under both reducing and non-reducing conditions. Subsequent studies suggest that CD203a migrates as a homodimer of ~260 kDa under non-reducing conditions and a 130 kDa monomer under reducing conditions (Petersen et al. 2007) from preparations of Porcine alveolar macrophages.CD203a is expressed widely in macrophage populations with notably high levels on alveolar macrophages (Petersen et al. 2007, Hwang et al. 2015), it is not expressed on monocyte populations (McCullough et al. 1997, Hwang et al. 2015).SWC1a, expressed at very much higher levels on monocytes than mature macrophages and CD203a (SWC9), expressed exclusively on mature tissue macrophages have been used as markers of monocyte-macrophage differentiation (Sanchez et al. 1999).
  • 反应物种: Pig
  • 应用: FACS, IHC-Fr, IP
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: PM18-7
  • 同位型: IgG1
  • 靶点名称: CD203a
  • 抗原物种: Pig
  • Brand:
CAT.NO. : ARG22652
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Product Details
概述
产品描述Mouse Monoclonal antibody [PM18-7] recognizes CD203a
This antibody recognizes Porcine CD203a, originally clustered as SWC9 at the Second International Swine CD Workshop (Dominguez et al. 1998) and later identified as the Porcine homologue of Human ecto-nucleotidepyrophosphatase / phosphodiesterase 1 or ENPP1 (Petersen et al. 2007).
Mouse anti Pig CD203a was originally reported to immunoprecipitate two bands, one of ~;205 kDa and one of ~130 kDa (Dominguez et al. 1998) under both reducing and non-reducing conditions. Subsequent studies suggest that CD203a migrates as a homodimer of ~260 kDa under non-reducing conditions and a 130 kDa monomer under reducing conditions (Petersen et al. 2007) from preparations of Porcine alveolar macrophages.
CD203a is expressed widely in macrophage populations with notably high levels on alveolar macrophages (Petersen et al. 2007, Hwang et al. 2015), it is not expressed on monocyte populations (McCullough et al. 1997, Hwang et al. 2015).
SWC1a, expressed at very much higher levels on monocytes than mature macrophages and CD203a (SWC9), expressed exclusively on mature tissue macrophages have been used as markers of monocyte-macrophage differentiation (Sanchez et al. 1999).
反应物种Pig
应用FACS, IHC-Fr, IP
宿主Mouse
克隆Monoclonal
克隆号PM18-7
同位型IgG1
靶点名称CD203a
抗原物种Pig
抗原Porcine alveolar macrophages.
偶联标记Un-conjugated
应用说明
应用建议
应用推荐稀释比
FACS1:25 - 1:200
IHC-FrAssay-dependent
IPAssay-dependent
应用说明FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purification with Protein A.
缓冲液PBS and 0.09% Sodium azide.
抗菌剂0.09% Sodium azide
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
检测图片 (1)
  • ARG22652 anti-CD203a antibody [PM18-7] FACS image

    Flow Cytometry: Pig alveolar macrophages stained with ARG22652 anti-CD203a antibody [PM18-7] followed by Goat anti-Mouse IgG (FITC).

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