anti-CD235a antibody [HIR2] (PE)
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CAT.NO. : ARG53799
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Product Details
概述
| 产品描述 | PE-conjugated Mouse Monoclonal antibody [HIR2] recognizes CD235a |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS |
| 特异性 | The clone HIR2 recognizes N-terminal portion of glycophorin A (and weakly of glycophorin B). Its antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells. HLDA VII; WS Code 70299 |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | HIR2 |
| 同位型 | IgG2b |
| 靶点名称 | CD235a |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide (Human, N-terminal) |
| 偶联标记 | PE |
| 別名 | MN; GPErik; MNS; GPA; GPSAT; PAS-2; MN sialoglycoprotein; CD235a; HGpMiV; CD antigen CD235a; HGpMiXI; Sialoglycoprotein alpha; HGpSta(C); Glycophorin-A |
应用说明
| 应用建议 |
| ||||
|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化说明 | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| 缓冲液 | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| 抗菌剂 | 15 mM Sodium azide |
| 稳定剂 | 0.2% (w/v) high-grade protease free BSA |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | GYPA |
| 全名 | glycophorin A (MNS blood group) |
| 背景介绍 | CD235a (Glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to glycophorin B (GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, an Streptococcus adhesin. |
| 生物功能 | Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV). [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody |
| 预测分子量 | 16 kDa |
| 翻译后修饰 | The major O-linked glycan are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH (about 78 %) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH (17 %). Minor O-glycans (5 %) include NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH NeuAc-alpha-(2-8)-NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH. About 1% of all O-linked glycans carry blood group A, B and H determinants. They derive from a type-2 precursor core structure, Gal-beta-(1,3)-GlcNAc-beta-1-R, and the antigens are synthesized by addition of fucose (H antigen-specific) and then N-acetylgalactosamine (A antigen-specific) or galactose (B antigen-specific). Specifically O-linked-glycans are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta-(3-1)-GalNAc-alpha (about 1%, B antigen-specific) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta (1 %, O antigen-, A antigen- and B antigen-specific). |
检测图片 (1)
ARG53799 anti-CD235a antibody [HIR2] (PE) FACS image
Flow Cytometry: Human peripheral blood stained with ARG53799 anti-CD235a antibody [HIR2] (PE).
参考文献
克隆号文献
Proteomic analysis of ERK1/2-mediated human sickle red blood cell membrane protein phosphorylation.
WB, IP / Human
Terminal differentiation and loss of tumorigenicity of human cancers via pluripotency-based reprogramming.
A novel fluorescence-based method in forensic science for the detection of blood in situ.
ICC/IF / Human
[Immunophenotyping study on the blood cells of the patients with paroxysmal nocturnal hemoglobinuria].
Flow cytometric analysis of human bone marrow perfusion cultures: erythroid development and relationship with burst-forming units-erythroid.
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