anti-CD25 antibody [OX-39]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [OX-39] recognizes CD25Mouse anti Rat CD25 antibody, clone OX-39 recognizes the alpha chain of rat CD25, otherwise known as IL-2 receptor alpha, a ~55 kDa type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla.Mouse anti Rat CD25 antibody, clone OX-39 has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde).Mouse anti Rat CD25 antibody, clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts (Paterson et al. 1987).
  • 反应物种: Rat
  • 应用: ELISA, FACS, IHC-Fr, IHC-P, IP
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: OX-39
  • 同位型: IgG1
  • 靶点名称: CD25
  • 抗原物种: Rat
  • Brand:
CAT.NO. : ARG23023
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Product Details
概述
产品描述Mouse Monoclonal antibody [OX-39] recognizes CD25
Mouse anti Rat CD25 antibody, clone OX-39 recognizes the alpha chain of rat CD25, otherwise known as IL-2 receptor alpha, a ~55 kDa type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla.Mouse anti Rat CD25 antibody, clone OX-39 has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde).Mouse anti Rat CD25 antibody, clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts (Paterson et al. 1987).
反应物种Rat
应用ELISA, FACS, IHC-Fr, IHC-P, IP
宿主Mouse
克隆Monoclonal
克隆号OX-39
同位型IgG1
靶点名称CD25
抗原物种Rat
抗原Stimulated Rat T cells
偶联标记Un-conjugated
別名IL-2-RA; IL-2 receptor subunit alpha; CD25; TCGFR; TAC antigen; IL2R; CD antigen CD25; Interleukin-2 receptor subunit alpha; IL-2R subunit alpha; p55; IL2-RA; IDDM10
应用说明
应用建议
应用推荐稀释比
ELISAAssay-dependent
FACS1:10 - 1:50
IHC-FrAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
应用说明IHC-P: PLP fixation is recommended.
FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purification with Protein G.
缓冲液PBS and 0.09% Sodium azide
抗菌剂0.09% Sodium azide
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 25704 Rat IL2RA

Swiss-port # P26897 Rat Interleukin-2 receptor subunit alpha

基因名称Il2ra
全名interleukin 2 receptor, alpha
背景介绍The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]
生物功能Receptor for interleukin-2. [UniProt]
研究领域Immune System antibody; Pre-B Cell Marker antibody
预测分子量31 kDa
检测图片 (6)
  • ARG23023 anti-CD25 antibody [OX-39] IHC-Fr image

    Immunohistochemistry: Rat lymph node cryosection stained with ARG23023 anti-CD25 antibody [OX-39] followed by HRP-conjugated Goat anti Mouse IgG1 as a detection reagent. (Low power).

  • ARG23023 anti-CD25 antibody [OX-39] IHC-Fr image

    Immunohistochemistry: Rat lymph node cryosection stained with ARG23023 anti-CD25 antibody [OX-39] followed by HRP-conjugated Goat anti Mouse IgG1 as a detection reagent. (Medium power).

  • ARG23023 anti-CD25 antibody [OX-39] IHC-Fr image

    Immunohistochemistry: Rat lymph node cryosection stained with ARG23023 anti-CD25 antibody [OX-39] followed by HRP-conjugated Goat anti Mouse IgG1 as a detection reagent. (High power).

  • ARG23023 anti-CD25 antibody [OX-39] IHC-Fr image

    Immunohistochemistry: Rat lymph node cryosection stained with ARG23023 anti-CD25 antibody [OX-39], red in A and Mouse anti Rat CD8, green in B. C is the merged image with nuclei counterstained blue using DAPI. (Low power).

  • ARG23023 anti-CD25 antibody [OX-39] IHC-Fr image

    Immunohistochemistry: Rat lymph node cryosection stained with ARG23023 anti-CD25 antibody [OX-39], red in A and Mouse anti Rat CD8, green in B. C is the merged image with nuclei counterstained blue using DAPI. (Medium power).

  • ARG23023 anti-CD25 antibody [OX-39] IHC-Fr image

    Immunohistochemistry: Rat lymph node cryosection stained with ARG23023 anti-CD25 antibody [OX-39], red in A and Mouse anti Rat CD8, green in B. C is the merged image with nuclei counterstained blue using DAPI. (High power).

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